摘要
目的利用微波辅助提取技术从竹叶柴胡中提取总黄酮。方法考察了影响提取率的因素，包括提取溶剂、物料粒度、微波时间、功率、固液比、pH值，并采用正交实验设计进行优化。结果确定了微波辅助提取竹叶柴胡总黄酮的最佳条件：60％乙醇为提取溶剂，竹叶柴胡粒度为120目以上，pH值7～8，按1：30的料液比，微波功率为640W下辐照3min．总黄酮提取率约为8．5％，与热回流提取2h相比提取率多出16．1％。结论微波辅助提取不仅提取率高，而且提取时间大大缩短，是竹叶柴胡中总黄酮快速高效的提取方法。
Aim To extract total flavonoids from Herba Bupleuri using microwave-assisted extraction. Methods Factors influencing the extraction percentage were examined, including extraction solvent, material grade size, microwave time and power, solid-liquid ratio and pH. The orthogonal experimental design was also be used to optimize the condition. Results The optimized conditions of microwave-assisted extraction were as follows：the extraction solvent was 60% alcohol; the sample was above 120 Meshed; pH was 7 - 8 ; solid-liquid was 1 ： 30 ; the power of extraction irradiation was 640W and the extraction time was 3min. The extraction yield of total flavonoids was 8.5%, 16.1% higher than that of heat recirculation of 2hours. Conclusion Microwave-assisted extraction is a rapid and efficient extraction technique for total flavonoids in Herba Bupleuri.
出处
《解放军药学学报》
CAS
2008年第4期311-313,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
微波辅助提取
槲皮素
竹叶柴胡
正交实验设计
HPLC
Microwave assist extraction
Total flavonoids
Bupleurum marginaturn Wall. Ex DC. , Herba Bupleuri
Orthogonol experimental design
HPLC
