摘要
目的从家蝇蛹血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽,然后经过研磨、离心和AKTATM purifier层析等步骤,将家蝇蛹抗菌肽进行分离纯化,采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经Resource S阳离子柱层析,测定280nm处紫外吸收值,在保留体积为9.4ml处出现1个紫外吸收峰,经检测对弓形虫速殖子有杀伤作用,再将其用于Superdex G75凝胶柱层析后,测定280nm处紫外吸收值,出现6个紫外吸收峰,发现第6个峰具有抗弓形虫作用。结论家蝇蛹血淋巴中存在抗弓形虫作用的抗菌肽。
Objective Isolate and purify the antimicrobial peptides with anti-Toocoplasma activity from the haemlymph of Musca damestica pupa. Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTATM purifier. Then the antimicrobial peptides with anti-Toa：oplasma activity were selected by haemacytometry and MTT colorimetric method. Results It was found that there was a ultraviolet absorption peak at 280 nm when retention volume was 9.4 ml through Resource S cationic exchange column chromatography, which had anti- Tozoplasma activity. This protein was collected and purified further with Superdex G75 gel column chromatography. And there were six ultraviolet absorption peaks at 280 nm through Superdex G75 gel column chromatography, and the sixth one had anti- Toxoplasma activity. Conclusion There was an antimicrobial peptide with anti-Toacoplasma activity in the haemlymph of M. domestica pupa.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2008年第4期309-311,共3页
Chinese Journal of Vector Biology and Control
基金
山西省高校科技研究开发项目(200613011)
山西省科技攻关项目(2006031087-04)
关键词
家蝇蛹
抗菌肽
抗弓形虫
纯化
Musca domestica pupa
Antimicrobial peptides
Anti-Toacoplasma
Purification
