摘要
目的研究复方罗布麻片中罗布麻叶的提取工艺。方法以槲皮素含量为指标,以乙醇浓度、乙醇体积和提取时间为考察因素,应用L9(34)正交试验优化罗布麻叶的提取工艺,并用高效液相色谱法测定复方罗布麻片中槲皮素的含量。色谱条件为:Kromasil C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.5%磷酸溶液(27∶73),检测波长为371nm,流速为1.0mL/min,柱温为30℃。结果用30倍85%乙醇,在水浴中回流2h。槲皮素在0.9630～0.0803μg范围内具有良好的线性关系,r=0.9998(n=7),平均回收率为99.46%。结论本工艺提取完全,并获得了满意的测定结果。
Objective To study the extraction process of kendir leaves in compound kendir leaves tablets. Method The optimum extraction process was selected by the orthogonal design L9（3^4）, with the factors of the concentration of ethanol, the volume of ethanol and the time of extraction, the content of quercetin as the evaluating criteria detected by HPLC. Kromasil Cis column （250 mm × 4.6 mm, 5 μm） was used. The mobile phase was acetonitrile-0.5% phosphoric acid （27：73）, detection wavelength was 371 nm, the flow rate was 1.0 mL/min, and the column temperature was 30℃. Results The best extraction process of kendir leaves was as follows： 85% ethanol, 30 times volume of ethanol, extracting for 2 hours in boiling water. Quercetin showed a good linearity in the range of 0.963 0-0.080 3 μg, r=0.999 8 （n =7）. The average recovery of quercetin was 99.46%. Conclusion The extraction process was entirely, and the results of determination are satisfactory.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第8期57-59,共3页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
复方罗布麻片
罗布麻叶
槲皮素
提取工艺
compound kendir leaves tablets
kendir leaves
quercetin
extraction process
