摘要
采用测序和单链构象多态(Single-strand conformation polymorphism,SSCP)的方法,分析了PRKAB2(Protein kinase,AMP-activated,beta 2 non-catalytic subunit)基因在5个优质肉鸡纯系和3个杂交配套系中的遗传分布、遗传变异及群体杂合性等群体遗传信息,并分析了PRKAB2基因对屠宰性状及肉质性状的遗传效应。结果发现该基因的CDS区域非常保守,仅在外显子1的406bp处发生点突变,由T→C。野生型A基因可显著提高活重、屠体重、腿肌重和腹脂重(P<0.05),同时也会提高肉的嫩度。
SSCP （Single-strand conformation polymorphism, SSCP） technique was applied in this study to analyze the population genetic information about genetic distribution, variation and heterozygosity of PRKAB2（Protein kinase, AMP-activated, beta 2 non-catalytic subunit） gene in five purelines and three crossbreds, The genetic effects of PRKAB2 gene on fiber density and partial carcass traits were also analyzed. Only one point mutation T→C at base position 406 located among the first exon of the whole CDS was found in the whole CDS.The wild-type allele A had significant positive effects on live-weight, carcass-weight, leg-muscle-weight and abdomen-fat-weight （P〈0.05）, and had positive effects on meat tenderness.
出处
《遗传》
CAS
CSCD
北大核心
2008年第8期1033-1038,共6页
Hereditas（Beijing)
基金
四川省科技厅十一五攻关项目“肉鸡配套系选育”(编号:2006-YZGG-19)资助~~
