摘要
目的研究细胞外基质受体Ⅲ(CD44)及其配体透明质酸(HA)在角膜碱烧伤修复过程中的作用。方法制作角膜碱烧伤大鼠模型,免疫组织化学方法观察CD44及HA在角膜碱烧伤后的表达变化,以正常大鼠和机械性划伤大鼠角膜为对照。结果CD44及HA在正常及机械性伤大鼠角膜仅少量表达;碱烧伤模型中烧伤后12h即可见表达增多,同时角膜鳞状上皮细胞、翼状细胞出现CD44。结论在角膜碱烧伤过程中CD44及HA对角膜的重新上皮化可能有积极作用。
Objective To investigate the expression of CD44 and hyaluronan in the corneal of alkaline-burned-rat （ABR） model and to reveal the function of them in the case of corneal rehabilitation. Methods The ABR model of the corneal was first developed, monitor the expression of CD44 and hyaluronan at the time point of 12 h,24 h,3 d,7 d,14 d after the injury was monitored by the means of immunohistochemical analysis. Results In the corneas of control teams, CD44 was little only expressed only in the basal column cells and the stromal kerotocytes at a low level. At the time point of 12 h after injury, increaseding expression was observed could be seen in the basal cells, and the expression also occureds in the flat cells and wing cells. The maximum predominant expression can be seen at the 3-7 d after the injury. At the 14 d when the epithelium had rehabilitated, the expression decreased in the basal column cells and stromal kerotocytes and disappeared in the flat cells and the wing cells, The expression pattern of HA is the same as CD44. Conclusion CD44 and it＇s receptor HA play important role in the course of the recovery of corneal alkaline burn. They participate inperform the adhesion of the cell-cell and cell-matrix, the epithelial migration and rehabilitation, and can facilitate help the corneal reepithelization.
出处
《福建医科大学学报》
2008年第4期313-315,共3页
Journal of Fujian Medical University
基金
山东省卫生厅青年基金资助项目(2007QZ017)
