摘要
对我国五大淡水湖日本沼虾100个野生个体的线粒体细胞色素氧化酶亚基I(COI)部分序列进行了测定和分析,经比对获得578bp核苷酸片段,发现49个变异位点,得到35个单倍型,包括7个共享单倍型,各群体都具有较好的单倍型多态性和核苷酸多态性,其中鄱阳湖群体遗传多样性相对最高。AMOVA分析表明,五群体间总遗传分化系数Fst=0.3187(P<0.05),群体间具有较高的遗传分化。MEGA3.1软件计算五群体的Kimura2-paramter遗传距离,洞庭湖群体和巢湖群体之间的遗传距离最远为0.0191,巢湖群体和洪泽湖群体之间的遗传距离最近为0.0051。以同属胖掌沼虾(Macrobrachium inflatum)为外群分别构建了NJ和UPGMA系统树,结果显示洞庭湖和鄱阳湖为一族群,太湖、巢湖和洪泽湖为一族群。
Oriental river prawn Macrobrachium nipponense is natively distributed in brackish and fresh waters, and can be found in the most parts of China, Japan, Korea, Vietnam and Myanmar. In China, the resource quantity of this species is very large in lower and middle reaches of the Yangtze River, especially in the five largest freshwater lakes. This species is a commercially important freshwater prawn species in China and Japan. But in recent years, the natural populations genetics and resource quantity of M. nipponense have declined rapidly due to overfishing, degradation and fragmentation of its natural habitats. Studying the distribution of genetic diversity and genetic structure in wild and culture stocks is important for developing conservation strategies. As an important and useful polymorphic DNA marker, mitochondrial cytochrome oxidase subunit Ⅰ（COI） gene was used to analyze the genetic diversity and genetic structure of five natural populations of M. nipponense, each of from the five largest freshwater lakes of China, i. e. Poyang Lake （PY）, Dongting Lake （DT）, Taihu Lake （TH）, Chaohu Lake （CH） and Hongze Lake（HZ）. Total gene DNA was extracted from the telson of the 20 individuals from each of five populations. A fragment of the COI gene was amplified using the general primers LCO1492 and HCO 2198, and sequenced on the ABI3730 sequencer. The result shows that forty nine variable nucleotide positions are detected in analyzed 578 bp COI sequences, and 35 haplotypes are defined among five populations, but only seven shared haplotypes are observed. Each population has good haplotypic diversity and nucleotide diversity, and among them PY shows the best genetic diversity. The fixation indices （Fst） analyzed by AMOVA of ARLEQUIN Version 3. 1 totalled to 0. 3187 （P〈0. 05）, which shows significant genetic fixation among the five populations. The Kimura 2-paramter genetic distance calculated by the MEGA version 3. 1 between the population of PY and CH is highest, up to 0. 0191, while
出处
《水产学报》
CAS
CSCD
北大核心
2008年第4期517-525,共9页
Journal of Fisheries of China
基金
上海市科委基础重大项目(06DJ14003)
上海市水产办项目(2005-06)
上海市重点学科建设项目(Y1101)
关键词
日本沼虾
细胞色素氧化酶亚基Ⅰ
遗传多样性
中国五大淡水湖
Macrobrachiurn nipponensec
cytochromec oxidase subunit Ⅰ
genetic diversity
the five largest freshwater lakes in China
