摘要
近年来,随着水产经济动物遗传育种研究的迅速发展,对染色体制备技术的要求越来越高.十足类甲壳动物染色体的研究开展较早,但进展却相当缓慢,自一百多年前,Carnoy首次报道了褐虾和滨蟹[1]的染色体,迄今为止已研究过的种类仅约70多种[2-6].
The Chromosomes of Macrobrachium nipponense had been prepared using the materials of testis or embryo with the chromosome low-osmosis method or cell low-osmosis method. After 5.5h , the bivalents at meiotic diplotene were appeared. The comparison of the two methods for two different materials showed that the morpha of the chromosome of testis using the low-osmosis method was better than that of using the cell low-osmosis method. Contrarily, the low-osmosis was better than the cell low- osmosis for the morpha of the chromosome of embryo. The bivalents at meiotic diplotene were rod-shaped, and centromere position could be identified easily at that time. The diploid chromosomes numbers were 104. The chromosome formula was N=11M＋26SM＋4ST＋11T. The technique of the chromosome preparation of Macrobrachium nipponense was preliminarily discussed as well.
出处
《实验生物学报》
CSCD
北大核心
2005年第5期456-460,共5页
Acta Biologiae Experimentalis Sinica
基金
浙江省重点攻关项目(2003C22012)
