摘要
目的研究前S1抗原与其他乙型肝炎表面标志物的相关性及其临床意义。方法ELISA法和定量PCR法对290份慢性乙型肝炎患者血清进行了HBVpreS1-Ag、HBsAg、HBsAb、HBeAg、HBeAb、HbcAb、HBV-DNA及丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)联合检测。结果在HBsAg(+)、HBeAg(+)、HBcAb(+)组中前S1抗原阳性所占比例为71.01%,显著高于HBsAg(+)、HBeAb(+)、HBcAb(+)组30.97%和HBsAg(+)、HBcAb(+)组41.07%(P<0.05);ALT、AST升高率均有显著性意义。结论前S1抗原与其他乙型肝炎表面标志物有一定相关性,在实际工作中两种方法可协同检测,相互补充。
Objective To evaluate the relationship and clinical value between Pre S1-Ag and other markers in serum of chronic hepatitic B patients.Methods HBV preS1-Ag, HbsAg, HbeAg,HbeAb,HbcAb,ALT, AST and HBV-DNA were detected by ELISA method and PCR-Flourescence quantity using serum samples of 290 cases with HBsAg（ ＋ ） as patient group and 50 cases with HBsAg （ - ） as comparison. Results the positive rate of Pre S1-Ag was 71.1 in nBsAg（ ＋ ）, nBeAg（ ＋ ）, nBcAb（ ＋ ） group, which is significant statistically compared with HBsAg（ ＋ ）, HBeAb（ ＋ ）, HBcAb（ ＋ ） group （ P 〈 0.05）. Conclusion The detection of hepatitis B virus Pre S1-Ag have relativity with other markers in serum of chronic hepafific B patients and can substitute hepatitis B markers detection.
出处
《中国实验诊断学》
2008年第7期894-896,共3页
Chinese Journal of Laboratory Diagnosis
