摘要
目的 了解乙肝患者HBVDNA含量及HBVDNA阳性患者用药前后的疗效观察。方法应用荧光PCR扩增病毒核酸免疫技术定量检测 6 5 0 0例血清标本的HBVDNA含量。结果 荧光定量PCR的特点与定性PCR主要区别在于荧光PCR即时反映扩增过程 ,采用内标法全封闭减少污染 ,足够灵敏的自动化仪器对荧光的收集和分析能达到对原始模板量定量检测。结论 用此方法可较准确、及时地为临床提供科学的诊断依据 ,并对HBVDNA阳性病人用药前后提供可靠数据。
Objective To discern the content of HBV DNA of the patient infected with HBV and to study curative effect of medicine. Methods Hepatitis B virus nucleic acid amplification(PCR) Fluorescence kits were used to quantify HBV DNA of 6500 specimens. Results There are many differences between fluorescence HBV PCR and qualification process. The internal control and close down can lessen pollution and the sensitive automaton can quantify determine. Conclusion This method can offer scientific basis for diagnosis and for medicine cure in time.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2001年第S1期121-122,共2页
Chinese Journal of Microbiology and Immunology
关键词
PCR免疫技术
HBV
DNA
定量分析
Hepatitis B virus
PCR fluorescence technique
Quantify HBV DNA
