摘要
目的:研究姬松茸发酵保健饮液的制备方法并对其免疫调节活性进行评价。方法:将姬松茸液态深层发酵液浓缩后,与菌丝体提取液进行混合,再经精滤、调配、装瓶、杀菌、冷却等工序制得姬松茸保健饮液。采用昆明种小鼠经口灌胃方式给与5、10和30mg/kg·d三种多糖剂量的保健饮液,通过测定免疫器官重量、腹腔巨噬细胞吞噬鸡红细胞指数、鸡红细胞作免疫源的溶血素含量及小鼠外周血中淋巴细胞酶标记染色测定、2,4-二硝基氟苯诱发的迟发型变态反应来评价该保健饮液对小鼠免疫功能的影响。结果:姬松茸发酵保健饮液能增强小鼠的胸腺指数和脾指数,提高腹腔巨噬细胞的吞噬功能和溶血素含量,增强2,4-二硝基氟苯所致的迟发型变态反应,其最适作用剂量为10mg/kg·d。结论:姬松茸发酵保健饮液可以提高小鼠非特异性免疫、特异性免疫和细胞免疫功能。
Objective： To determine the fermented oral liquid production procedure of Agaricus blazei and to evaluate its immunomodulating activity. Methods： Concentrated submerged cultural broth of Agaricus blazei was blended with extract of the mecylium. And the Agaricus blazei oral liquid （AbOL） was prepared through the processes of fine filtration, preperation, bottling, sterilization, and cooling. Kunming mice were used to detect the effects of the oral liquid on immune activities （i.p.） with different polysaccharide dosages （5 mg/kg·d, 10 mg/kg·d and 30 mg/kg·d）. The measured items included immune organ weight, chicken red blood cell phagocytic index of macrophage cell of abdominal cavity, hemoglobin amount analysis with chicken red blood cell as immune source, ELISA of lymphocyte in mice peripheral blood and DNFB-induced DTH. Results： Agaricus blazei oral liquid has the function to increase spleen and thymus index, phagocytic function of macrophage cell and hemoglobin amount. And it can enhance DNFB-induced delayed allergy of mice. The optimal dosage of polysaccharide is 10 mg/kg·d. Conclusion： Agaricus blazei oral liquid has the funtion of promoting unspecific immunity, specific immunity and cellular immunity of mice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期405-407,共3页
Food Science
基金
辽宁省教育厅项目(20040292)
沈阳市科学计划项目(1063307-1-04)
2007年辽宁省自然基金项目
关键词
姬松茸
保健饮液
多糖
免疫调节活性
Agaricus blazei
healthy care oral liquid
polysaccharide
immunomodulating activity
