摘要
研究了培养时间、初始pH值、乙醇浓度、二次补糖等因素对冬虫夏草菌丝生长和产糖的影响,得出了最佳培养时间为6d,初始pH为5.5,乙醇浓度为1.0%,二次补糖为复合碳源葡萄糖+甘露糖+半乳糖(3:4:2)1.2g/100ml,发酵液胞外多糖产量和菌丝干重最高分别可达5949.6mg/L和17.35g/L。功能性虫草饮料以添加枸杞+党参(2:1)15g/100ml发酵的配伍最佳。最优饮料配方:发酵液:水=70:30、蔗糖7%、柠檬酸0.1%、稳定剂0.15%。
The effects of culture time, initial pH, ethanol concentration and secondary supplementing sugar on the biomass and the polysac, charides production of Cordyceps sinensis were studied. The results showed:the optimum culture time 6d, initial pH 5.5, ethanol thickness 1.0% and secondary supplementing sugar Glu+Man+Gal(3:4:2)1.2g/100ml. The maximal output of polysaccharides and the mycelium weight reached 5949.6mg/L and 17.35g/L respectively. The optimum functions obtained when cordycepic beverage was added with Lycium barbarum and Codonopsis pilosula (2:1) 15g/100ml. The best beverage formula is fermentative fluid:water=-70:30, sucrose 7%, citric acid 0.1% and stability 0.15%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第2期205-209,共5页
Food Science
基金
河南科技学院重点科研基金资助项目
关键词
冬虫夏草
深层培养
多糖
饮料
Cordyceps sinensis
submerged culture
polysaccharides
beverage