摘要
建立了用甲醇提取,并用WatersHBL固相萃取小柱进行净化,高效液相色谱(HPLC)法测定芦荟干粉中芦荟甙的含量。使用C18柱,以甲醇:1%醋酸水溶液(体积比为1:1)为流动相,采用紫外检测器在359nm处对样品中的芦荟甙进行测定,外标法进行定性定量分析。测定结果表明,芦荟甙在0～100mg/L时其峰面积与进样质量的线性相关系数达到0.9999,测定的相对标准偏差(RSD)为2.5%～3.2%(n=6)。试样加标回收率为98.1%～101%。该方法的最小检测浓度为0.02mg/kg。测定结果显示:该方法快速、灵敏、可靠,具有简便,重现性好的特点。
Aloin was extracted from dry aloe powder with methanol, further purified by solid phase extraction column, and then determined by high performance liquid chromatograph （HPLC）. The HPLC operation conditions were as follows： C18 column （4.6 mm×150 mm, 5μm）, methnaol-1% acetic acid water （1：1, V/V） as mobile phase, and UV detector wavelength 359 nm. The quantitative and qualitative analyses were performed with external standard method. The results of determination showed that there is a good linear calibration curve in the range from 0 to 100 mg/L （r=0.9999） with relative standard deviations of 2.5% to 3.2% （n=6）. The recovery rates of spiked aloin range from 98.1% to 101%, and the detection limit is 0.02 mg/kg. This method is rapid, sensitive, simple, accurate and repeatable.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期322-324,共3页
Food Science
关键词
芦荟干粉
芦荟甙
固相萃取
高效液相色谱法
dry aloe powder
aloin
solid phase extraction
high performance liquid chromatography
