摘要
目的:确定清开灵注射液及其各中间体中氮的存在形式及含量。方法:使用日立L-8500氨基酸自动分析仪,填充分析柱(2.6 mm×50mm),日立2622 SC树脂,缓冲液流速0.4 mL·min^(-1),茚三酮显色液流速0.3 mL·min^(-1),柱温程序升温:57℃(0 min)～65℃(36 min)～57(50 min),反应温度130℃。结果:清开灵注射液及其各中间体中的氮主要以游离型或结合型氨基酸的形式存在,其质量分数分别为水解液59.56%,板蓝根提取液24.88%,四混液41.84%,六混液13.49%,八混液14.63%;清开灵注射液及其各中间体中均含有结合型氨基酸,其质量分数分别为水解液9.33%,板蓝根提取液15.07%,四混液16.85%,六混液19.94%,八混液19.55%,清开灵注射液18.73%。结论:清开灵注射液中总氮主要来自水牛角和板蓝根。
Objective： To analyze characterization and determination of nitrogen in the preparation of Qingkailing injection and its intermediate products. Method： Hitich amino acid auto analyzer was used, with the packed analysis column （2. 6 mm × 50 mm） and the type of was Hitich 2622 SC resin. The speed of buffer solution and ninhydrin colorimetric solution were 0.4 mL·min^- 1 and 0. 3 mL· min^-1 respectively. Program heating was used for controlling column temperature, from 57℃ （0.0 min） to 65℃ （36 min） to 57 （50 min）. The reaction temperature was set at 130℃. Result： Free and binding amino acid the existenceare the main form of nitrogen is amino acid in Qingkailing injection and its intermediate products. The total contents of amino acid in the preparation of Qingkailing injection and its intermediate products, including hydrolyzed solution which is made from neutralization of Concha Marga- ritifera solution extracted by diluted sulfuric acid and Cornu Bubali solution extracted by diluted sodium hydroxide, aqueous solution of Radix Isatidis extract, 4-blended solution, 6-blended solution and 8-blenede solution, were 59. 56%, 24. 88%, 41.84%, 13.49, 14. 63% respectively. The type of bonded amino acid was founded in the preparation of Qingkailing injection and its intermediate products, including hydrolyzed aqueous solution of Radix Isatidis extract, 4-blended solution, 6-blended solution and 8-blenede solution, and the contents were 9. 33%, 15.07%, 16.85%, 19.94% and 19.55%, respectively. Conclusion： The main resource of the total nitrogen was Bubalus bubalis L. and Isatis indigotica Fort.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第14期1673-1676,共4页
China Journal of Chinese Materia Medica
基金
北京市科委项目(H030230170130)
关键词
清开灵注射液
各中间体
氮
氨基酸
Qingkailing injection
intermediate products
nitrogen
amino acid
