摘要
目的建立复方毛冬青注射液细菌内毒素的含量测定方法。方法将复方毛冬青注射液作倍比稀释,采用动态浊度法通过干扰试验和验证试验考察其最佳稀释倍数,测定复方毛冬青注射液中细菌内毒素的含量。结果将样品作80倍稀释可以有效消除对鲎试验的干扰,检测内毒素平均回收率均在50%～200%范围内。结论使用动态浊度法定量检测复方毛冬青注射液中的细菌内毒素是可行的。
Objective To develop an assay for determining bacterial endotoxin（BET） content in Maodongqing Compound Injection （MCI）. Methods The dilution radio of Maodongqing Compound Injection was optimized and BET content was determined by turbidimetric kinetic method. Results Dilution ratio of Maodongqing Compound Injection at 1 ： 80 did not interfere with the Limulus test. The average recovery of BET was between 50 % and 200 %. Conclusion The method is proved to be accurate, sensitive and suitable for the determination of BET content in Maodongqing Compound Injection.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第4期291-293,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
2006年广州市中医药中西医结合科研课题(2006B25)
关键词
复方毛冬青注射液
动态浊度法
细菌内毒素
Maodongqing Compound Injection
Turbidimetric - kinetic method
Bacterial endotoxin
