摘要
用果针入土后４５天左右的花生未成熟子叶为外植体，在含高浓度６－苄基腺嘌呤（ＢＡ）的培养基中，置于约１８００ｌｕｘ光照和２６±１℃条件下，培养２１～２８天，能有效地诱导产生幼芽。其中５号培养基（ＭＳ基本培养基＋ＢＡ２０ｍｇ／１＋ＡＤ１００ｍｇ／ｌ＋蔗糖３％＋琼脂０．８５％），芽诱导率达９２．５％。截取诱导苗带叶腋茎段转接在８号（ＭＳ＋ＢＡ４０ｍｇ／ｌ＋ＹＥ０．０１％）、９号（ＭＳ＋ＢＡ３０ｍｇ／ｌ＋ＹＥ０．０１％＋ＡＤ５０ｍｇ／ｌ）两种培养基中，置于约１８００ｌｕｘ光照和２３～２８℃条件下，培养２１天开始出现丛生芽，４２天后（８）号和（９）号两种培养基均有９５％以上的外植体分化出丛生芽，继而长成无根小苗。平均每个外植体分化出１５个以上小芽，多的达５０个以上。
Buds were induced in vitro effectively employing immature cotyledons as explant after pegging into the soil for about 45 days,in a medium with high content BA and in a light of 1800lux and at 26±1℃after 21～28 days. Among the culture system,the No. 5 medium,(MS+BA 20mg/l+AD 100mg/l+sugar 3%+Agar 0.85%), produced a shooting rate at 92.5% of the explants. As the stem sections with nodes were used and transplanted in two mediems, No. 8(MS+BA 40mg/l+YE 0.01%) and No. 9 (MS+BA 30mg/l+YE 0.01%+AD 50mg/l), multiple buds were observed after culture for 21days; and 42 days later, more than 95% of explants produced multiple buds with over three buds which then grew further into seedings without root.There were over 15 shoots per explant and the better explant had over 50 buds.
出处
《花生科技》
北大核心
1997年第3期1-4,共4页
基金
福建省花生育种攻关项目
关键词
花生
子叶
丛生芽
组织培养
Peanut
cotyledon
tissue culture
bundle buds
