摘要
目的观察湖北民族药红活麻有效部位对小鼠皮肤移植排斥反应的影响。方法采用小鼠同种异体皮肤移植模型，通过皮肤移植物存活时间、受体脾淋巴细胞增殖实验、细胞因子分泌水平测定及CD4^＋CD25^＋T细胞亚群分析研究红活麻有效部位抗皮肤移植排斥反应的作用及机制。结果与模型对照组平均皮肤移植物存活时间（14．4±0．9）d比较，红活麻有效部位中剂量组、高剂量组皮肤移植物存活时间分别为（25．9±1．8）和（41．2±2．8）d，提示红活麻有效部位呈剂量依赖性延长小鼠皮肤移植物存活时间；中剂量组、高剂量组对非特异性刺激（ConA）的脾淋巴细胞增殖反应强度均低于模型对照组（P〈0．05）；同时高剂量组抑制脾细胞分泌IL-2、IFN-1水平明显（P〈0．01），刺激IL-10的分泌水平明显（P〈0．01）。结论红活麻有效部位通过刺激淋巴细胞表面CD4^＋CD25^＋分布，调节IL-2、IL-10和IFN-1等细胞因子的合成，抑制T淋巴细胞转化功能，诱导宿主细胞免疫耐受，从而有效抑制皮肤移植排斥反应。
Objective To investigate the anti-rejection effects of the active component from Honghuoma on skin transplantation model and the underlying mechanism. Methods The anti-rejection effects and the underlying mechanism were studied by detecting survival time of allografts, T cells proliferation, cytokines secretion and subsets of CD4 ^＋ CD25^ ＋ T cells in recipients. Results Compared with aUografts in the control group whose survival time was （14.4 ±0.9） days,the survival time in middle- and high-dose Honghuoma groups was （25.9 ± 1.8 ） and （41.2 ± 2.8 ） days, respectively. The active components of Honghuoma could prolong the survival time of allografts. Meanwhile, the active components suppressed T cell proliferation （ P 〈 0.05 ） and IL-2, IFN-7 secretion （ P 〈 0.01 ）, however, increased IL-10 （ P 〈 0.01 ） and percentage of CD4^ ＋ CD25^ ＋ T cells significantly in a dose-dependent manner. Conclusion The active components from Honghuoma shows obviously antl-rejection effects on the skin transplantation model by stimulating CD4^＋ CD25 ^＋differentiation, modulating IL-2, IFN-7 and IL-10 production, and inducing immune tolerance.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第7期940-942,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30672655）
关键词
皮肤移植
红活麻
免疫耐受
排斥反应
Skin transplantation
Honghuoma
Immune tolerance
Rejection effect
