摘要
目的:建立超高效液相色谱-电喷雾-四级杆-飞行时间串联质谱联用技术(UPLC-ESI-Q-TOF-MS)研究红禾麻提取物中的主要化学成分。方法:采用Agilent Eclipse Plus C18RRHD色谱柱(2.1 mm×100 mm,1.8μm);以0.1%甲酸水溶液-0.1%甲酸乙腈溶液为流动相进行梯度洗脱;流速0.25 m L·min-1,柱温40℃,进样量1μL。采用电喷雾离子源(ESI),负离子模式进行检测,运用Data Analysis软件,并结合保留时间、精确相对分子质量和相关文献进行数据分析。结果:通过结合对照品及文献报道的质谱数据对红禾麻提取物中化学成分进行分析,共分析鉴定13个化学成分,包括4个儿茶素类,3个苯丙素类和6个黄酮类,其中新绿原酸、隐绿原酸、绿原酸、芦丁、异槲皮苷、木犀草苷、山柰酚-3-O-芸香糖苷、紫云英苷和槲皮苷均首次在该植物中发现。结论:研究中建立的检测方法简便、准确,为快速鉴定分析化学成分提供一种有效的分析方法,并比较全面地阐明了红禾麻提取物中的化学成分,为其质量控制和药效物质基础研究奠定一定的基础。
Objective:To establish a method for content identification of main chemical compositions in Laportea bulbifera extract by using ultra performance liquid ehromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS).Method :The chromateographic separation was performed on Agilent Eclipse Plus C18RRHD (2.1mm × 100mm,1.8μm)with 0.1%formic acid solution-0.1%formic acid acetonitrile solution as mobile phase for gradient elution.The flow rate was 0.25mL·min^-1;column temperature was set at 40℃ and injection volume was 1μL.Electrospray ionization (ESI)was applied and operated innegative ion mode.By using Data Analysis software combined with retention time,exact relative molecular weight and reference data,the structures of the monitored chemical compositions were identified.Result:Thirteen chemical compounds were identified by molecule mass information,reference data and present standards,including 4catechins,3phenylpropanoids and 6 flavonoids.Among them,neochlorogenic acid,cryptochlorogenic acid, chlorogenic acid,rutin,isoquercitrin,luteoloside,kaempferol-3-O-rutinoside,astragalin and quercitrin were first found in L.bulbifera.Conclusion:The established detection method was convenient and accurate,providing an analytical approach for rapidly identifying and analyzing the chemical compounds of L.bulbifera extract,and laying a foundation for its quality control as well as further studies on clarifying the material basis of its efficacy.
作者
唐娟
吴耽
陈思颖
李莹
李靖
巩仔鹏
李维维
兰燕宇
王永林
TANG Juan;WU Dan;CHEN Si-ying;LI Ying;LI Jing;GONG Zi-peng;LI Wei-wei;LAN Yan-yu;WANG Yong-lin(Guizhou Provincial Key Laboratory of Pharmaceutics,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,School of Pharmaceutial Science,Guizhou Medical University,Guiyang 550004,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第24期67-72,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81560693)
贵州省科技厅科技创新人才团队建设项目(黔科合平台人才[2016]5613)
贵州省高层次创新型人才培养计划项目(黔科合平台人才[2016]5677
贵阳市科研创新团队项目(筑科合同[2017]30-29号)
