摘要
基于酶催化和荧光毛细分析法(FCA)开发了一种微量、快速测定葡萄糖的新方法(GE-FCA)。优选的实验条件为:反应时间15min;反应温度30℃;磷酸盐缓冲液(pH6.0),HRP浓度和GOD浓度分别是200和150U/L;线性范围0.1～4mg/L;检出限0.070mg/L。GE-FCA对血液中葡萄糖进行测定,其回收率在98.9%～103.8%之间。与其它方法相比,GE-FCA法操作简单,而且试样用量少(仅为18μL),节省了酶试剂用量,实验成本低,易于普及推广。
A method was developed for the quick determination of glucose using fluorescence capillary analysis（FCA）.The experimental conditions were as follows：reaction time,15 min;temperature：30 ℃;pH of phosphate buffer solution：6.0;the concentrations of herseradish peroxidase（HRP） and glucosse oxidase（GOD） were 200 U/L and 150 U/L,respectively.The linear range of FCA was 0.1-4 mg/L,the relative standard deviation（RSD） was 〈1.2%,and the detection limit of glucose was 0.07 mg/L.The method has been applied for the determination of glucose in blood,the recovery was 98.9%-103.8%.Compared with the other methods,the FCA had a more simple operation.A tiny sample volume was required（only 18 μL）,so it has the advantage for saving the enzyme reagent.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第7期991-994,共4页
Chinese Journal of Analytical Chemistry
基金
四川大学214人才引进振光计划基金(No0082204127067)资助项目
关键词
荧光毛细分析法
血液
葡萄糖
葡萄糖氧化酶
辣根酶
Fluorescence capillary analysis,blood,glucose,glucosse oxidase,herseradish peroxidase
