摘要
目的探讨4种内植物材料对表皮葡萄球菌黏附及形成生物被膜的影响。方法表皮葡萄球菌培养、分离纯化、药敏试验及生物被膜形成鉴定,将钛合金、钴铬钼、超高分子量聚乙烯(UHMWPE)、国产骨水泥(PMMA)制成圆片状试样,分别与表皮葡萄球菌悬液共同培养5d;试样表面黏附的细菌经胰酶消化、制成细胞悬液、定量接种培养及菌落计数,观察4种试样表面黏附的细菌数量;真空干燥法制备试样,扫描电镜(SEM)观察4种试样表面生物被膜形成情况。结果此菌株为表皮葡萄球菌,对半合成青霉素耐药,可形成生物被膜;细菌计数显示UHMWPE试样表面细菌数量最多,为(24.96±1.459)×105/CFU;其次为国产PMMA,为(17.44±1.883)×105/CFU;金属合金表面细菌数量较少,钛合金为(0.424±0.065)×105/CFU,钴铬钼为(0.382±0.075)×105/CFU;UHMWPE、国产PMMA与其他各组比较,均P值<0.05;SEM观察发现UHMWPE试样表面有较多生物被膜形成,钛合金及钴铬钼表面细菌聚集成团,国产PMMA表面黏附许多单个细菌菌落。结论4种内植物材料对表皮葡萄球菌黏附及形成生物被膜有影响,表皮葡萄球菌容易在UHMWPE表面黏附、形成生物被膜。
OBJECTIVE To investigate the in vitro effect of 4 kinds of implant materials on the biofilm formation of Staphylococcus epidermldis. METHODS S. epidermidis was cultured, purified and identified. Susceptibility test was done for S. eplderrnidls and the ability to produce biofilm was proven. The test samples were made into wafer shape for titanium alloy, Co-Cr-Mo alloy, ultrahigh molecular weight polyethylene （UHMWPE） and homemade polymethyl methacrylate （PMMA）. S. epidermldis was cultured with 4 kinds of test samples for 5 days respectively. Bacteria adhering on surfaces of 4 kinds of test samples were dissolved with trypsin, and then diluted into bacterial suspensions. Each bacterial suspension was inoculated quantitatively and CFU were counted. Biofilm on surfaces of 4 kinds of test samples prepared by vacuum drying method was observed with SEM. RESULTS The strain proven to be S. epidermidis, was resistant to semisynthetic penicillins, and could produce biofilm. CFU count showed that CFU were the most on the UHMWPE surface and the number of CFU were（24. 965±1. 459）× 10^5. CFU were （17. 445±1. 883） × 10^5 on the PMMP surface. （0. 424±0. 065） × 10^5 CFU were discovered on the surfaces of titanium alloy and （0. 382 ±0. 075）× 10^5 CFU on Co-Cr-Mo alloy. When each group compared with UHMWPE and domestic PMMA respectively, all P value was 〈 0. 05. Some biofilms were found on the UHMWPE surface by SEM. S. epldermidis was singling on the PMMA surface and agglomerated on the titanium alloy and Co-Cr-Mo alloy surfaces. CONCLUSIONS Four kinds of implant materials have an effect on the adherence and biofilm formation of S. epldermidis, and S. epidermidis could produce biofilms on the UHMWPE surface in vitro.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第7期924-927,共4页
Chinese Journal of Nosocomiology
关键词
内植物
感染
表皮葡萄球菌
材料
生物被膜
Implant
Infection
Staphylococcus epidermidis
Material
Biofilm
