摘要
目的探讨基质金属蛋白酶2(MMP2)和转化生长因子β1(TGF-β1)在肾透明细胞癌组织中的表达及其意义。方法应用半定量逆转录-聚合酶链反应(RT-PCR)检测43例肾透明细胞癌患者癌与癌旁组织MMP2和TGF-β1的表达水平。结果在不同的病理分级和临床分期的43例肾透明细胞癌组织中,MMP2表达水平均显著高于癌旁组织;癌与癌旁MMP2表达的差值随临床分期的升高而呈上升趋势。TGF-β1在肾透明细胞癌组织中的表达阳性率为32.56%(14/43),而癌旁组织均无表达;TGF-β1表达阳性的肾透明细胞癌病理分级和临床分期均处于Ⅰ或Ⅱ级(期)。结论MMP2和TGF-β1在肾透明细胞癌组织过度表达参与其浸润生长,侵袭转移等病理过程,对其检测为预后判断提供了帮助。
Objective To study the expression and significance of MMP2 and TGF-β1 in human suprarenal epithelioma tissues. Methods Expressions of MMP2 and TGF-β1 gene from 43 human suprarenal epithelioma patients＇ carcinoma and tumor-adjacent tissues were detected by semiquantitative reverse transcription polymerase chain reaction （RT-PCR）. Results The expression of MMP2 in the carcinoma tissues was significantly higher than that in the tumor-adjacent tissues in all differencent pathological grade and clinical stage. There was a tendency of increased expression of MMP2 in the tumor-adjacent tissues with increased clinical stage. The expression rate of TGF-β1 in human suprarenal epithelioma tissues was significantly higher than that in the corresponding tumoradjacent tissues （32.56% vs. 0%）. TGF-β1 was expressed in grade and staage Ⅰ or Ⅱ suprarenal epithelioma tissues. Conclusion Overexpressed MMP2 and TGF mRNA might contribute to the infiltrating growth and invasive metastasis of human suprarenal epithelioma, which may be useful in predicting the prognosis.
出处
《江苏医药》
CAS
CSCD
北大核心
2008年第7期682-684,共3页
Jiangsu Medical Journal
