摘要
从16周龄朗德鹅肝脏组织提取总RNA,采用反转录-多聚酶链式反应(RT-PCR)方法扩增出1条长约280bp的cDNA片段。序列分析结果表明,扩增出的cDNA片段与固醇调节元件结合蛋白1(SREBP-1)基因有较高的同源性,并且该片段包含着1个bHLH功能区。同时该片段包含1个明显的亲水区和1个明显的疏水区,二级结构富含α-螺旋,这与其功能相符。
Total RNA from Landes goose liver of 16 weeks old was extracted. The cDNA segment about 282 bp of Langdes goose was amplified by the reverse transcription-polymerase chain reaction（RT-PCR）. Sequence analysis showed that there is a high similarity between the fragment of cDNA and sterol regulatory element binding protein-1（SREBP-1） gene containing a bHLH function region. Meanwhile, the segment evidently has a hydrophilic region and a hydrophobic region, and the secondary structure richly contains α-helix, which accords with its function.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第7期994-999,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省十一五育种攻关项目(2006YZGG-20)
四川省教育厅重点专项(2004D001)
关键词
朗德鹅
固醇调节元件结合蛋白1基因
生物信息学分析
Landes goose
sterol regulatory element binding protein-1 gene
bioinformatics analysis
