摘要
目的:观察丹酚酸B对大鼠局灶性脑缺血再灌注损伤后脑组织bcl-2和bax蛋白表达的影响。方法:健康雄性Wistar大鼠24只,随机分成3组:假手术组,模型组,丹酚酸B组,每组8只。丹酚酸B组预先给药,1次/d,连续7d。末次给药后1h除假手术组外其他两组大鼠线栓法制备大脑中动脉局灶性缺血再灌注模型,缺血2h,再灌注24h。实验结束时采用免疫组化方法,通过病理的图文分析系统检测各组大鼠脑组织bcl-2和bax蛋白表达量以及二者比值。结果:模型组大鼠脑组织bcl-2和bax蛋白表达较假手术组明显增高,以后者更为显著,二者比值失调;丹酚酸B组能继续升高bcl-2蛋白表达量,同时降低bax蛋白表达,恢复二者比例。结论:丹酚酸B能上调bcl-2蛋白表达以及下调bax蛋白表达,并恢复二者比值,这可能是丹酚酸B防治缺血性脑血管疾病的作用机制之一。
Objective： To study the effects of sallvianolic acid B on the expression of bcl -2 and bax proteins in rats with focal cerebral ischemia reperfusion injury, and analyze the mechnism of action of sallvianolic acid B in preventing and treating ischemic cerebrovascular disease. Methods： 24 healthy male Wistar rats were randomly divided into 3 groups with 8 rats in each group ： sham - operation group, model group, sallvianolic acid B groups. The latter group infused cor- responding drugs, once daily for 7 days. Then the intraluminal suture technique was used to establish the models of middle cerebral artery occlusion （MCAO） in rats except sham - operation group, for 2h of occlusion and 24h of reperfusion. At the end of the experiment, the immunohistochemistry and pathological image analytical technique were used to monitor the expression of bcl - 2 and bax proteins after the focal cerebral ischemia reperfusion in brain tissue of the three groups, and ratios of bcl - 2 and bax were compared. Results ： In modle group, the expression of bcl - 2 or bax proteins in brain tissue of rats was higher than those in sham - operated, the latter was increased more obviously ; and ratio of both expres- sions was in imbalance. In saUvianolic acid B group, compared with model group, the expression of bcl- 2 protein was increased, while the expression of bax protein was reduced; and ratio of both expression was recovered. Conclusion： Sallvianohc acid B could up - regulate the expression of bcl - 2 protein, down - regulate the expression of bax protein simultaneously and recover the ratio of bcl - 2/bax, which might be one of mechanisms of sallvianolic acid B preventing and treating ischemic cerebrovascular disease.
出处
《中华中医药学刊》
CAS
2008年第7期1475-1476,共2页
Chinese Archives of Traditional Chinese Medicine
基金
天津市卫生局科技基金资助课题(04KY36)
