摘要
对利用琼脂糖-纤维蛋白平板法测定纳豆激酶活力的方法进行了改进,对测定纳豆激酶活力的标准曲线的规律进行了研究。研究发现,当尿激酶活力在200U/mL～10000U/mL时,尿激酶活力的常用对数lgU与对应的溶圈面积S呈线性相关,其表达式的斜率与孵育时间、孵育温度和平板厚度有关。通过试验确定了测定纳豆激酶活力的最佳孵育时间为15h,此时的酶活测定标准曲线为:lgU=0.0052S+b,b值的确定方法为:将2种标准尿激酶液的酶活力和对应的溶圈面积分别带入标准曲线lgU=0.0052S+b中,分别求出其对应的修正系数b1、b2,以b=b1+2b2为标准曲线lgU=0.0052S+b的修正系数,利用此法计算酶活力的相对误差为0.22%。
The method of measuring the activity ofnattokinase with agarose-fibrin plate was improved. The standard curve which was used to measure the activity of nattokinase was studied. The relationship of the common logarithm of the urokinase activity and the dissolving circle submitted the regressionship while the urokinase activity range is 200U/ml-10000U/ml. The slope of the equation is related to the incubation time, the in cubation temperature and the plate height The best incubation time of measuring the nattokinase activity is 15 h, and the standard curve is ： lgU=0.0052S＋b. The value of b can be calculated as follows： put the activity value and dissolving circle of two kinds of urokinases into the equation of b= b1＋b2 lgU=0.0052S＋b, then b1 and b2 were worked out, and the value of b=b1＋b2/2 The relative error is 0.22%.
出处
《中国酿造》
CAS
北大核心
2008年第4期77-80,共4页
China Brewing
