摘要
通过研究反应速率与底物浓度之间的关系,确定大麦多酚氧化酶(PPO)的米氏常数Km为1.89×10-3mol/L。利用双因素全面性实验测定大麦PPO作用的最适条件,结果表明,80℃、pH8.4为大麦PPO最适作用条件。建立分光光度法测定大麦发芽过程中PPO活性变化的方法,最适测定条件为酶添加量<5mL,反应时间4min,检测波长450nm。PPO在沸水中处理9min即可有效抑制其活性。
Through the relation between the reaction rate and the concentration of substrate, the determinate Michaelis constant Km of the polyphenol oxidase （PPO） of barley was 1.89 × 10^-3mol/L Utilizing the double factors general experiment to determine the optimal assay conditions and the optimal conditions are as followings： the temperature 80℃ and pH 8.4, the enzyme solution 5 ml, reaction for 4 min, 450 nm absorption wave length. In the boiling stabilization aspect, treating 9 min can effectively inhibit the activity of PPO. According to the results as before, the activity of PPO was determined during the germination of barley by spectrometry.
出处
《中国酿造》
CAS
北大核心
2008年第4期35-38,共4页
China Brewing
基金
国产大麦指纹图谱库的建立(2004ln-fj-004)
关键词
多酚氧化酶
大麦
米氏常数
polyphenol oxidase
barley
Michaelis constant
