摘要
目的探讨妊娠期硬膜外给予利多卡因和/或静置24h大鼠血清神经并发症高发的可能病因。方法性成熟雌性非妊娠SD大鼠43只、妊娠20～28dSD大鼠44只,分别随机分为四组。非妊娠大鼠:利多卡因组(NL组,2%利多卡因),血清组(NS组,静置24h大鼠静脉血清),利多卡因+血清组[NLS组,2%利多卡因+血清(1:1)],对照组(NC组,生理盐水);妊娠大鼠:利多卡因组(PL组,2%利多卡因),血清组(PS组,静置24h大鼠静脉血清),利多卡因+血清组[PLS组,2%利多卡因+血清(1:1)],对照组(PC组,生理盐水)。每次给药0.2ml,重复3次。用药后0、2、5、10、20、30和40min,观察并记录大鼠的SBP及运动阻滞情况。24h后脊髓取材,固定切片染色后,光镜下观察脊髓形态学改变。结果与给药前比较,NS、NC、PS、PC组的SBP及NS、NC组运动功能差异均无统计学意义。NL、NLS、PL、PLS组给药后,SBP显著下降(P<0.05),并产生不同程度的运动阻滞(P<0.05),但组间差异无统计学意义。NL、NLS、PL和PLS组给药40min后,SBP和运动功能均恢复到给药前水平。给药后10min内及20～30min,NL、PL组运动阻滞分别强于NLS、PLS组(P<0.05),但NL、NLS、PL和PLS组间差异无统计学意义。脊髓形态学检查各组差异无统计学意义。结论无论是否妊娠,硬膜外应用0.2ml2%利多卡因或大鼠血清,不会导致大鼠脊髓神经损伤的发生。
Objective To investigate the possible etiological factor of higher neurologic complications in pregnants given epidural lidocaine or serum. Methods Forty-three SD rats of sexmatured, forty-four rats with 20 to 28 days pregnant were randomly divided into 8 groups. Nonpregnant rats were assigned into control group （NC, saline）, lidocaine group （NL, 2% lidocaine）, serum group （NS, serum） and lido-caine-serum group[NLS, 2% lidocaine＋serum（1 ： 1）]. Pregnant rats were assigned into control group （PC, saline）, lidocaine group （PL, 2%lidocaine）, serum group （PS, serum） and lidocaine-serum group[PLS, 2% lidocaine ＋ serum（1： 1）]. 0.2 ml was administrated each time, which was repeated for 3 times. The motor block score and SBP of rats were observed at 0,2,5,10,20,30 and 40 min after each administration. The spinal cord （3 cm around the tip of catheter） was immediately removed at 24 h after the last injection for histological examination by light microscopy. Results There were no differences in SBP and motor block scores between group NC and PC or between group NS and PS. The SBP decreased and motor block scores increased in group NL and PL, but no significant difference was found between the two groups. The same phenomenon was found in group NLS and PLS. The SBP and motor block scores were recovered at 40 min after each epidural administration. At 20 and 30 min after each epidural injection, SBP of group NL was lower than that of group NLS, and SBP of group PL was lower than that of group PLS （P〈0.05）. Within 10 min and during 20-30 min after each epidural injection, the motor block score of group NL or PL was higher than that of group NLS or PLS,respectively （P〈0.05）. There was no significant difference in histological change of the spinal cord among all groups. Conclusion Whether pregnant or not, no neurologic impairments of epidural administration with 0.2 ml 2% lidocaine, serum or 2% lidocaine/serum （1：1） was found.
出处
《临床麻醉学杂志》
CAS
CSCD
2008年第6期515-517,共3页
Journal of Clinical Anesthesiology
