摘要
[Objective] The study aimed to identify woltberry （Lycium Linn.） germplasm resources at molecular level by analyzing the nrDNA ITS sequence. [Method] Genomic DNA from woltberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by specific primer, clone and sequencing. [Result] The nrDNA ITS sequences were obtained and then differentiated among three tested materials. [Conclusion] PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different woltberry germplasm resources.
ITS区序列指DNA基因内的转录间隔区序列，包括ITSl、ITS2及5．8S3个区段。ITS区序列测定分析能够鉴别同属植物相似种以及药用植物混淆种。笔者以枸杞属内不同种间ITS序列为切入点，对枸杞nrDNA ITS序列进行分析研究，为从分子水平鉴定枸杞品种提供参考。
基金
Supported by Natural Science foundation of Ningxia Hui Antonomous Region(NZ0769)~~
