摘要
A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol （saturated with water）, chloroform, Guanidine isothioeyanate were used as main extraction reagents. Polyphenolie compounds were removed effectively by added PVP into the extraction buffer solution. RNA was purified intensively by phenol, chloroform extraction, and ethanol deposition after deposited by LiCl. Both the results of formaldehyde denatured agarose gel eleetrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA. So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.
笔者采用改良CTAB法从富含多酚的无核荔枝幼胚中提取总RNA，并用甲醛变性琼脂糖凝胶电泳和紫外分光光度计对所提RNA质量进行检测，旨在为多糖、多酚含量较高的植物材料RNA的提取提供一种有效方法。
基金
Supported by the National“863”Import Program(AA001380)~~
