摘要
目的:探讨黄疸婴儿HCMV感染的诊断.方法:FQ-PCR检测248例黄疸婴儿和50例对照组患儿尿液中HCMV DNA,化学发光免疫分析法(CLIA)检测黄疸婴儿血清HCMV IgM抗体,在尿液HCMV DNA阳性黄疸婴儿中,比较血清HCMV IgM抗体阳性与阴性组HCMV DNA拷贝数的差异.结果:黄疸婴儿尿液HCMV DNA的阳性率为41.5%,对照组患儿阳性率为2.0%,两者有统计学意义(P<0.01).CLIA检测黄疸婴儿血清HCMV IgM抗体的阳性率为19.8%.在尿液HCMV DNA检测阳性的黄疸患婴儿中,IgM抗体阳性组的尿液HCMV DNA拷贝数显著高于阴性组(t=5.51,P<0.01).结论:FQ-PCR检测尿液HCMV DNA是早期诊断婴儿HCMV感染的敏感有效的方法.
AIM： To investigate the diagnosis of human cytomegalo virus （HCMV） infection in infants with jaundice.
METHODS： Urine specimens were collected from 248 infants with jaundice and 50 control infants without jaundice to detect HCMV DNA using fluorescence quantitative polymerase chain reaction （FQ-PCR）. Blood samples were collected from infants with jaundice to detect HCMV IgM antibody using chemi-luminescent immunoassay（CLIA）. Among HCMV DNA positive infants, quantitative levels of HCMV DNA were compared between HCMV IgM positive group and the negative group.
RESULTS： The FQ-PCR positive rate of the infants with jaundice （41.5%） was significantly higher than that of control infants （2.0%）（P 〈 0.01）. CLIA positive rate of the infants with jaun- dice was 19.8%. HCMV DNA of the HCMV IgM positive group were significantly higher thanthat of the negative group （t = 5.51, P 〈 0.01） CONCLUSION： FQ-PCR is a sensitive and effective test for early diagnosis of HCMV infection in infants.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第15期1704-1707,共4页
World Chinese Journal of Digestology
关键词
婴儿
黄疽
荧光定量PCR
化学发光免疫分析法
人巨细胞病毒
Infants
3aundice
Fluorescent quantitative polymerase chain reaction
Chemi-luminescent immunoassay
Human cytomegalovirus
