摘要
目的建立大鼠自体免疫性前列腺炎的模型。方法将32只SD大鼠随机分为4组,其中一组为对照组,其它三组为试验组,在第0天和第7天,进行皮内多点注射,分别给予20、40和80g/L的SD大鼠前列腺蛋白提纯液及完全弗氏佐剂(FCA),同时腹腔注射百白破疫苗。在第21天,腹腔注射大鼠前列腺蛋白提纯液。在第35天,处死所有大鼠,对大鼠前列腺解剖学、病理学、血液白细胞数、血清钙离子、IgG、睾酮(T)和双氢睾酮(DHT)等指标进行测定和研究。结果和阴性对照组相比,高剂量造模组的白细胞数明显增加,前列腺明显充血水肿,间质和腺腔有淋巴细胞和单核细胞浸润,表现出明显的慢性前列腺炎病理变化,其他两组不如高剂量组明显。高剂量模型组的钙离子浓度明显降低。结论多点多次皮内注射80g/L的大鼠前列腺蛋白提纯液与FCA的混悬液,腹腔注射百白破疫苗可成功地建立SD大鼠自体免疫性前列腺炎模型。
Dawley（SD） Objective To establish an animal model of autoimmune prostatitis in Spraguerats. Methods At the 0 and 7th day, male rats were immunized with a saline extract of male SD rat prostate glands（RPG） with the dosage of 20, 40 and 80g/L（intracutaneous） and Pertussis-Diphtheria-Tetanus vaccine 0.5 ml （peritoneal injection）. At 21st day, male rats were immunized with 0.5 ml saline extract of rat prostate glands. At 35th day, the rats were sacrificed, and the prostate specimens, calcium ion, testosterone（T） and dihydrotestosterone（DHT） were observed or determined. Results Compared with control group, Wt3C counts increased obviously in high-dosage model group, its interstitial tissue and glandular cavities were full of akaryocytes, lymphocytes and monocytes. The high-dosage model group appeared chronic inflammation response, calcium ion obviously decreased. Conclusion Thrice and multipoint injecting 80 mg/ml RPG with FCA and twice injecting 0.5 ml Pertussis-Diphtheria-Tetanus vaccine was an effective method for establishing autoimmune prostatitis model in SD rats.
出处
《实验动物与比较医学》
CAS
2008年第3期151-154,共4页
Laboratory Animal and Comparative Medicine
