摘要
小鼠卵泡卵母细胞体外培养过程中加入2 mmol/L 6-DMAP可抑制卵母细胞自发的染色质浓缩和生发泡破裂(GVBD)。源自超排的MⅡ期卵母细胞则能为6-DMAP所激活。hCG注射后18—19h的卵母细胞置于2 mmol/L6-DMAP的CZB溶液中培养0.5 h、1h、2h、3h,卵母细胞的激活率分别为26.1%、75.2%、75.8%、77.3%、;卵裂率分别为88.2%、73.2%、67.0%、58.4%。与乙醇激活法相比,6-DMAP处理引起了不同的孤雌激活类型。
Chromatin condensation and germinal vesicle breakdown (GVBD) in mouse cumulus free oocytes was blocked by 2 mmol/L of 6-DMAP in vitro. Mouse oocytes (MⅡ) from superovulated female mice could be activated by 6-DMAP. The activation rates of oocytes (18-19 h post hCG) cultured in CZB medium with 2 mmol/L 6-DMAP for 0.5、 1、 2、 3 h,
were 26.1%、 75.2%、 75.8%、 77.3% respectively (Table 1), and the cleavage rates of these activated oocytes were 88.2%、 73.2%、 67.0%、 58.4% respectively (Table 2). The parthenogenetic activation type induced by 6-DMAP was different from that produced by ethanol (Fig.1).
出处
《实验生物学报》
SCIE
CAS
CSCD
1997年第4期403-406,共4页
Acta Biologiae Experimentalis Sinica
关键词
小鼠
6-DMAP
卵母细胞
减数分裂
孤雌发育
Mouse. 6-Dimethylaminopurine. Germinal vesicle breakdown. Parthenogenetic activation.
