摘要
目的以腺样囊性癌肺高转移细胞株(adenoid cystic carcinoma cell clones highly me-tastatic to the lung,ACC-M)为研究对象,探讨ABCG2在ACC-M的表达情况及意义,为今后分离ACC-M的肿瘤干细胞提供一定的实验依据及理论数据。方法用流式细胞计数检测ABCG2在ACC-M的表达情况。结果流式检测显示ABCG2的表达率为8.64%±0.68。结论ACC-M细胞也象多数实体瘤一样含有SP细胞。在后续的研究中,可考虑利用SP细胞的特异性表型标记"AB-CG2",将SP细胞从肿瘤中分离出来,进一步探讨肿瘤干细胞的生物学特性。
Objective To investigate the expression and significance of ABCG2 in adenoid cystic carcinoma cell line（ACRE-M）, and to lay experimental basis for further isolating the tumor stem cells. Methods ACC-M tumor cells are detected and analysed the expression of surface antigens ABCG2 by flow cytometry. Results The expression rate of ABCG2 in cell surface of ACC-M isS. 64%±0. 68 by flow cytometry measuring. Conclusion The ACC-M cells also contain SP cells, as some cancer cells. In future studies, the phenotype “ABCG2” which labeled SP cells will be considered to be a specific marker to isolate SP cells from the tumor cells. Through the research of SP cells, we will further explore the biological characteristics of tumor stem cells.
出处
《贵州医药》
CAS
2008年第6期501-502,共2页
Guizhou Medical Journal
基金
贵州省科技基金资助项目[黔科合2004(3054)]
关键词
涎腺肿瘤
腺样囊性癌
肿瘤干细胞
Salivary tumor Adenoid cystic carcinoma Tumor stem cells
