摘要
从各地采集的土样中筛选对兰花炭疽病病原菌胶孢炭疽菌(Colletoichum gloeosporioides)具有拮抗活性的菌株。从土样中分离到720株菌株,初筛获得具有拮抗作用的菌株32株,经过复筛,选出1株具有较高拮抗活性的菌株8-59。对8-59菌株进行形态鉴定、生理生化特征鉴定和16S rDNA序列分析,菌株8-59与花域芽孢杆菌(Bacillus vallismortis)很相近。根据16S rDNA序列相似性分析,此菌株与花域芽孢杆菌标准菌株DSM 11031的16S rDNA序列相似度达99.49%。鉴定该菌株为花域芽孢杆菌。
A total of 720 strains were isolated from the soil and 32 strains which have antagonistic activity against Colletoichum gloeosporioides were screened via preliminary screening. A strain named 8 - 59 with a rather higher antagonistic activity was obtained after secondary screening. Thus the morphology characteristics, physiological and biochemical properties and 16S rDNA sequence of this strain were further studied. As the morphological characteristics and physiological and biochemical characteristics of the stain are similar to Bacillus vallismortis and the similarity of the 16S rDNA sequences between 8 - 59 strain and the type strain was up to 99.49%, the 8 - 59 strain was finally identified as Bacillus vallismortis.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2008年第3期64-68,共5页
Journal of Hebei Agricultural University
基金
“948”项目“花卉新品种创制技术引进与创新”(2006-4一C08)
国家“十一五”科技支撑计划课题“主要商品花卉优质高产新品种选育”之林业科技支撑专题“优质高产热带兰新品种选育”(2006BAD01A18)
关键词
兰花炭疽病
胶孢炭疽菌
拮抗细菌
筛选
鉴定
花域芽孢杆菌
orchids anthracnose
Colletoichum gloeosporioides
antagonistic bacteria
screening
identification
Bacillus vallismortis
