摘要
目的制备毛蚶提取物并分析其抗氧化活性。方法利用Superdex-75,Sephadex LH-20凝胶柱层析等方法从毛蚶中分离纯化具有抗氧化活性的组分P3,采用铁氰化钾,DPPH等方法测定P3的还原力及清除自由基能力,同时采用茚三酮法、蒽酮法、考马斯亮蓝结合法、薄层层析等方法对该组分进行了定性分析。结果与结论从毛蚶中分离得到了具有抗氧化活性的组分P3,经定性分析推测该活性组分为糖肽。
Objective To prepare active components from extract of Arca subcrenata Lischke and analyze its antloxidative activity. Methods The antioxidative component （P3）from Arca subcrenata Lischke was isolated by chromatography on Superdex -75 column followed by a Sephadex LH- 20 column, and antioxidative activities were assayed using potassium ferricyanide and DPPH methods, respectively. The characters of this component were determined by ninhydrin reagent, anthrone reagent, Coomassie Brilliant Blue G250 reagent as well as thinlayer chromatography. Results and Conclusion A component with strong antioxidative activity was isolated and identified as glycosylated peptide.
出处
《中国海洋药物》
CAS
CSCD
2008年第3期11-14,共4页
Chinese Journal of Marine Drugs
基金
青岛大学科研基金资助项目(No.QDDX-06-49)
关键词
毛蚶
抗氧化
分离
纯化
Arca subcrenata Lischke
antioxidation
isolatiom purification
