摘要
目的探讨应用较小剂量阿霉素间断静脉注射的方法制备扩张型心肌病模型的可行性。方法阿霉素(1～2mg/kg/1～2周,共8周)耳缘静脉注射,观察实验组图造模前后超声心动图的改变,用药结束3周后与对照组(生理盐水注射,共8周)组织形态进行比较。结果实验组动物死亡率与对照组无显著差异(P>0.05);阿霉素组的左室射血分数(LVEF)及左室短轴缩短率(FS)显著低于造模前(P<0.01),而左室舒张末内经(LVd)及左室收缩末内径(LVs)均显著高于造模前(P<0.01);组织病理显示模型组心肌细胞不均匀肥大,细胞浓染,局部心肌胞浆空泡形成符合扩心病表现。结论阿霉素诱导可成功诱导制备兔扩张型心肌病模型,个体化间断给药可减少动物死亡率,可作为研究治疗扩张型心肌病药物的实验动物模型。
Objective To approach the feasibility of preparing dialed cardiomyopathy animal models with adriamycin. Methods The adriamycin （1-2 mg/kg/1-2 w, 8 w） and normal saline （4 ml·kg^-1·-w^-1, 8 w） were intravenously injected into the tested group and the control group, respectively. Transthoracic echocardiogramphy was performed in the experiment rabbits at baseline and 11 w after administration. The left ventricular slices of those two groups were processed for hematoxylin-eosin stain in the 11th w. Results There were no significant difference for the mortal rate between the two groups （P〉0.05）. Comparing with the baseline, the ejection fraction （LVEF%） decreased from 80.0±3.1 to 55.4±6.5 （P〈0.01）, shortening fraction （FS%） decreased from 47.2±3.6 to 27.9±4.4 （P〈0.01）, end-systolic diameters （LVs） increased from 7.1±1.1 mm to 9.0±1.7 mm, end-diastolic diameters （LVd） increased from 11.3±2.1 mm to 14.1±2.3 mm （P〈0.01）. There were no significant difference for LVEF and FS before and after injection in the control group （P〉0.05）. Uneven hypertrophy and dark dyed of cardiac myocytes, cavitation of endochylema in some cardiac myocytes, which are similar to that found in dilated cardiomyopathy of human beings, could be observed in the histopathological slices of the experimental animals. Conclusion Adriamycin can be used to prepare the dilated cardiomyopathy models of rabbits. This model may be useful for the research, and to find effective drugs for the treatment of dilated cardiomyopathy.
出处
《今日药学》
CAS
2008年第3期80-82,共3页
Pharmacy Today
关键词
实验动物模型
兔
阿霉素
心肌病
experimental animal model
rabbit
Adriamycin
dilated cardiomyopathy
