摘要
目的 :探讨阿霉素诱导兔心肌病模型的可行性 ,并利用经胸超声心动图进行评估。方法 :2 0只兔每周静脉注射阿霉素 2mg·kg-1,共计 8周 (总剂量 16mg·kg-1) ,另 8只每周注射同等剂量的生理盐水作为对照组。基础状态 (未注药前 )、12周时分别进行兔超声心动图检查 ,分别测量左室舒张期末、收缩期末内径 ,室间隔和左室后壁厚度 ,以及射血分数和短轴缩短率。 12周时阿霉素组和对照组分别取 2只进行心肌病理切片检查。结果 :阿霉素组与基础状态相比 ,12周时左室舒张期末内径显著扩大(10 .5± 1.3和 15 .8± 2 .1mm ,P <0 .0 5 ) ,收缩期末内径显著扩大 (8.7± 1.0和 10 .3± 1.1mm ,P <0 .0 5 ) ,射血分数显著降低 (6 3.4± 8.4和 38.8±7.6 ,P <0 .0 5 ) ,短轴缩短率显著降低 (P <0 .0 1) ,室间隔和左室后壁厚度无显著性变化 (37.6± 7.6和 2 1.2± 6 .7,P >0 .0 5 ) ;对照组左室舒张期末、收缩期末内径、室间隔和左室后壁厚度、射血分数和短周缩短率均无显著性变化 (P >0 .0 5 )。阿霉素组病理结果显示了与人类阿霉素心肌毒性相似的改变 ,心肌细胞变性、线粒体和肌浆网水肿、细胞内空泡增多。结论 :静脉注射阿霉素可以诱导形成非缺血性心肌病模型 ,结果可靠 ,成功率高。
AIM : To determine the efficacy and reliability of adriamycin induced cardiomyopathy model in rabbits and to evaluate it by transthoracic echocardiography. METHODS : Twenty rabbits were given 2 mg·kg -1 of adriamycin intravenously once a week for 8 weeks (total dose, 16 mg·kg -1 ) to induce the cardiomyopathy model. Other eight rabbits received 2 ml·kg -1 of normal saline once a week for 8 weeks as control. The left ventricular dimensions, wall thickness, fractional shortening and ejection fraction were measured by transthoracic echocardiography on the 1st day as baseline and in the 12th week after treatment. 2 rabbits from each group were sacrificed for histological study in the 12th week. RESULTS : Compared with the baseline conditions, the value for the left ventricular diameter of the diastole (LVEDD) and that of the systole (LVESD) increased significantly, while the value for ejection fraction (EF) and the fractional shortening (FS) decreased significantly after treated with adriamycin ( P < 0.05 ). The values for interventricular septal thickness (IVST) and left ventricular posterior wall thickness (PWT) did not differ after treatment ( P BZ > 0.05 ). In the control group, no significant changes were found in all parameters between the 1st day and the 12th week ( P > 0.05 ). The characteristics of adriamycin cardiotoxicity in human patients such as cardiomyocytes degeneration, mitochondrial and sarcoplasmic reticular edema, numerous intracellular vacuoles were found in the driamycin treated group in histological study. CONCLUSION : Intravenous injection of adriamycin can be used to induce a reliable rabbit model of non ischemic dilated cardiomyopathy with a high success rate. Transthoracic echocardiography can be applied in monitoring the animal model’s formation.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第1期48-51,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
超声心动图
兔
阿霉素
心肌病
动物模型
echocardiography
rabbits
adriamycin
cardiomyopathy
animal models
