摘要
目的:观察电针"四关"穴对抑郁模型大鼠结肠组织诱导型一氧化氮合酶(iNOS)、谷胱甘肽过氧化物酶(GSH-Px)活性及一氧化氮(NO)含量的影响,探索电针保护结肠组织的作用机制。方法:将40只SD雄性大鼠随机分为正常组、模型组、药物组及电针组,每组10只,用慢性应激结合孤养的方式造模21d。在造模同时,药物组按1.8mg/kg给予氟西汀灌胃,1次/d,连续21d;电针组取"合谷"和"太冲",频率为1.5～2Hz,疏密波,电压9V,电流强度1～3mA,留针20min,1次/d,连续21d。紫外分光光度法检测大鼠结肠组织中iNOS、GSH-Px的活性及NO的含量。结果:与正常组比较,模型组大鼠结肠组织中iNOS活性、NO含量增加,GSH-Px活性降低(P<0.05);与模型组比较,电针、药物可降低抑郁模型大鼠结肠组织中iNOS的活性及NO的含量,提高结肠组织中GSH-Px的活性(P<0.05);电针组与药物组比较,两者间差异无显著性意义(P>0.05)。结论:电针"四关"穴具有保护抑郁状态下大鼠结肠组织功能的作用。
Objective To observe the effect of electroacupuncture （EA） on the activity of inducible nitric oxide synthase （iNOS）, glutathione peroxidase （GSH-Px） and the content of nitric oxide （NO） in colon tissue of depression rats, so as to reveal its mechanism in protecting colonic tissue under depression. Methods Forty male SD rats were equally randomized into normal, model, medication and EA groups. Depression model was established by using chronic unpredictable mild stress stimulation （forced ice-water swimming, electric shock, tail-clamping, etc） combined with lonely raising for 21 days. At the same time of modeling, Fluoxetine （1.8 mg/kg, intragastric perfusion） was given to the rats of medication group, once daily for 21 days. EA （1.5-2 Hz, 1 -3 mA） was applied to bilateral＂Hegu＂ （LI 4） and ＂Taichong＂ （LR 3） for 20 min, once daily for 21 days. The activity of iNOS, GSH-Px and the content of NO in colon tissue were detected by ultraviolet spectrophotometer method. Results In comparison with normal control group, iNOS activity and NO content in colon tissue increased significantly （P〈0.05）, and GSH-Px activity decreased considerably （P〈0.05） in model group. Whereas compared with model group, iNOS activity and NO content decreased significantly （P〈0.05）, and GSH-Px activity increased significantly in EA and medication groups. No signifi- cant differences were found between EA group and medication group in these 3 indexes （P〉0.05）. Conclusion EA of ＂Hegu＂ （LI 4） and ＂Taichong＂ （LR 3） can reduce stress induced increase of both iNOS activity and NO content, and decrease of GSH-Px activity in colon tissue in depression rats, which may contribute to its effect in protecting the digestive tract from injury under depression.
出处
《针刺研究》
CAS
CSCD
2008年第3期183-185,共3页
Acupuncture Research
