摘要
目的:对三种角膜接触镜与棘阿米巴原虫的黏附能力进行比较,观察加用无菌生理盐水反复吹打角膜接触镜表面,对清除接触镜表面棘阿米巴效果的影响。方法:取市售的三种角膜接触镜分为硬性透气性角膜接触镜(RGP)组、亲水性软性接触镜(SCL)组、彩色角膜接触镜组,并分别与棘阿米巴悬液共同培养16h,镜下观察比较棘阿米巴的黏附数量,并将彩色接触镜的有色区域和无色区域进行比较;加用无菌生理盐水反复吹打角膜接触镜表面后,比较接触镜表面残存的棘阿米巴数量。结果:彩色接触镜组的阿米巴黏附数量高于RGP组和SCL组(P<0.05).RGP组和SCL组之间差别无统计学意义(P>0.05)。彩色接触镜组其彩色区域的棘阿米巴的黏附数量高于无色区域(P<0.01).三组接触镜经吸管用无菌生理盐水冲洗后,棘阿米巴的黏附数量均有明显降低(P<0.01)。结论:与RGP组和SCL组的接触镜相比,彩色接触镜更易受到棘阿米巴原虫的黏附;加用无菌生理盐水反复吹打角膜接触镜表面可明显加强对接触镜表面棘阿米巴的清除效果。
AIM ： To compare the adherent ability of Acanthamoeba to three kinds of contact lens （CL） and survey the effect to remove the Acanthamoeba from the surface of contact lens after blowing with stroke-physiological saline solution （SPSS） repeatedly.
METHODS： Three kinds of contact lens were chosen in this experiment, including： rigid gas permeable （RGP） contact lens, soft contact lens （SCL） and colored contact lens. They were co-cultivated with Acanthamoeba suspension for 16 hours. Then the number of Acanthamoeba adhered on CL was compared. Comparison was also made between different zones of colored CL. We also compared the number of Acanthamoeba adhered on CL in three groups after blowing with SPSS.
RESULTS： The number of Acanthamoeba adhered to colored CL was more than RGP group and SCL group （P〈0. 05）. The differences between RGP group and SCL group had no statistical significance （ P〉0. 05）. In colored CL group, the number of Acanthamoeba adhered to the colored zone was more than the uncolored zone （P〈 0.01 ）. In all the three groups, after blowing with SPSS, there was statistically significant decrease of the number of Acanthamoeba adhered to CL （ P〈0.01 ）.
CONCLUSION： Compared with RGP group and SCL group, the colored contact lens was more vulnerable to be adhered by Acanthamoeba. After being blown by SPSS repeatedly, the effect to eliminate Acanthamoeba has been improved greatly.
出处
《国际眼科杂志》
CAS
2008年第6期1101-1103,共3页
International Eye Science
