摘要
目的:探讨白藜芦醇对缺氧诱导的视网膜血管内皮细胞增殖的影响。方法:用100μmol/LCoCl2模拟缺氧环境,以体外培养的人视网膜血管内皮细胞为模型,采用MTT法测定细胞增殖、SABC法检测血管内皮生长因子的表达,并以计算机图像分析仪进行分析,观察研究白藜芦醇对CoCl2诱导的视网膜血管内皮细胞增殖的影响。结果:白藜芦醇对CoCl2诱导的视网膜血管内皮细胞增殖具有显著抑制作用(P<0.01),且呈时间和剂量依赖关系;同时,各给药组均抑制VEGF的表达(P<0.01)。结论:白藜芦醇能抑制CoCl2诱导的视网膜血管内皮细胞增殖及VEGF的表达,将为视网膜新生血管疾病的药物治疗提供一条新思路。
AIM. To study the effects of resveratrol on the proliferation of human retinal vascular endothelial cells induced by cobalt chloride-simulated hypoxia in vitro.
METHODS： CoCI2 （100μmol/L） was used to simulate hypoxic condition, and human retinal vascular endothelial cells were cultured in vitro as model. The cell proliferation was determined by MTT method; SABC method was employed to test the expression of vascular endothelial growth factor（VEGF） ; and computer image analyzer was used to process data. The effects of resveratrol on the proliferation of vascular endothelial cells were observed.
RESULTS： Resveratrol inhibited the proliferation of human retinal vascular endothelial cells induced by CoCl2 in a dose- and time-dependent manner in vitro, meanwhile VEGF expression in all groups which were administered medicine was down-regulated. Both kinds of inhibitive effects of resveratrol were statistically significant （P〈0.01）.
CONCLUSION： Resveratrol can significantly inhibit the proliferation of human retinal vascular endothelial cells and the expression of VEGF, which may provide a new approach for prevention and treatment of retinal neovascular diseases.
出处
《国际眼科杂志》
CAS
2008年第6期1087-1090,共4页
International Eye Science
关键词
白藜芦醇
视网膜血管内皮细胞
细胞增殖
血管内皮细胞生长因子
resveratrol
retinal vascular endothelial cells
cell proliferation
vascular endothelial growth factor
