摘要
宫颈癌细胞(HELA)、人胚肾细胞(HEK293)和中华仓鼠卵巢细胞(CHO)3种细胞系在用于哺乳动物细胞悬浮培养的锥底生物反应器(FCB)、平底生物反应器(FB)和常规机械搅拌式生物反应器(STR)中进行培养和冷模实验。结果表明:FCB中HELA细胞的最高密度比FB高27%,HEK293和CHO细胞的最高密度分别比STR高38%和43%。FCB的混合时间平均比FB短27%,比STR长35%;kLa比FB和STR分别高83%和97%。
Three cell lines of HELA, HEK293 and CHO were cultured in a novel inverted frusto-conical bottom bioreactor （FCB）, flat bottom bioreactor （FB） and conventional mechanism stirred bioreactor （STR）, respectively. Highest cell densities in FCB for HELA,HEK293 and CHO cells were 27% ,38% and 43% higher than that in FB,STR and STR, respectively. Additionally, mixing time and ki.a of three types of bioreactors were determined. Average mixing time of FCB is 27% shorter than that of flat bottom one, while 35% longer than that of STR. Average kLa of FCB is 83% and 97% higher than that of FB and STR, respectively.
出处
《华东理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2008年第3期338-341,共4页
Journal of East China University of Science and Technology
基金
上海市科委重点项目(064319033)
上海市重点学科建设项目资助(B505)
关键词
生物反应器
动物细胞悬浮培养
混合时间
氧传递系数
bioreactor
mammalian cell suspension c ulture
mixing time
oxygen transfer coefficient
