摘要
【目的】构建白菜(Brasscia campestris ssp.chinensis)分子转录图谱。【方法】以矮脚黄白菜自交系(97-3-2)和白蔓菁芜菁自交系(001-24),以及以两者为亲本杂交建立的F6重组自交系群体为材料,在莲座期取嫩叶提取RNA并反转录成cDNA,采用cDNA-AFLP技术,利用256对cDNA-AFLP引物对亲本97-3-2、001-24之间的多态性进行检测,筛选出56对多态性引物用于F6群体分析,通过对cDNA-AFLPs分子标记的遗传分析构建图谱。【结果】构建了一个包括164个cDNA-AFLPs分子标记的分子转录图谱。该图谱由13个连锁群组成,图谱总长度为1401.2cM,标记间平均图距9.7cM。【结论】这是第一份利用cDNA-AFLP标记构建的白菜分子转录图谱。
【Objective】Chinese cabbage-pak-choi （Brassica campestris L. ssp. chinensis Makino） transcriptome map was constructed with cDNA-AFLP techniques. 【Method】The inbred line Aijiaohuang 97-3-2,the inbred line Baimanjing 001-24 of turnip [B. campestris ssp. rapifera （Matzg.） Sinsk ] and 183 F6 （recombinant inbred population） plants were used as experimental materials. cDNAs were synthesized from total RNA extracted from young leaves at rosette stage. 256 pairs of cDNA-AFLP primers were used to detect the polymorphisms between parents Aijiaohuang 97-3-2 and Baimanjing 001-24. 56 pairs of cDNA-AFLP primers with high polymorphisms were screened from 256 pairs of primer by DNA-AFLP techniques. The genetic diversity of parents and 183 F6 progenies was detected by 56 pairs of cDNA-AFLP primers. The segregation and distribution of cDNA-AFLPs molecular marker were analyzed to construct transcriptome map among parents and F6 plants. 【Result】 A total of 164 cDNA-AFLPs marker loci were mapped into 13 linkage groups which covered 1 401.2 cM with an average distance of 9.7 cM. 【Conclusion】It is the first transcriptome map of Chinese cabbage using cDNA-AFLP technique.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第6期1735-1741,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30560087)
关键词
白菜
CDNA-AFLP
转录图谱
Brassica campestris ssp. chinensis
cDNA-AFLP
Transcriptome map
