摘要
目的：建立LC-ESI-MS/MS法测定服药后人体血浆氯沙坦及E3174的浓度。方法：MI-CROMASS Quattro Micro API型液质联用仪,色谱柱为Inertsil ODS-3C18柱（2.1mm×150mm,5μm,Ja-pan）,流动相为0.1%甲酸-乙腈（3070,V/V）,流速为0.2mL/min,进样体积为5μL,柱温为40℃,样品室温度为15℃。结果：氯沙坦线性范围为2.2~1085.0ng/mL,E3174线性范围为2~1000.0ng/mL,氯沙坦及E3174最低检测限低于0.5ng/mL,方法灵敏、稳定、特异性高,并成功地应用到人体氯沙坦及E3174药代动力学研究。结论：该方法简便、准确、重复性好,可以准确定量服药后人体血浆氯沙坦及E3174的浓度。
AIM： To establish a LC-ESI-MS/MS method for determination of losartan and E3174 in hu- man plasma. METHODS： LC-MS/MS（MICROMASS Quattro Micro API） and Inertsil ODS-3 C18 column （2.1 mm× 150 mm, 5 pro） were used in the study. The column temperature was set at 40 ℃. 0.1% ammonium formate-acetonitrile （30： 70, V/ V） was used as mobile phase and the flow rate was 0.2 mL/min. The sample room temperature was set at 15 ℃ and the injection volume was 5 μL. RESULTS： losartan and E3174 were linear range from 2.2 - 1085.0 ng/mL and 2 - 1000.0 ng/mL respectively, the limitation of detection for losartan and E3174 were about 0.5 ng/mL, the method was of high sensitivity, stability and specificity and has been used for pharmacokinetic study of losartan and E3174 /n vivo successfully. CONCLUSION： the method is simple, accurate, repetitive for the determination of losartan and E3174 in human plasma and suitable for the application in pharmacology.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2008年第4期438-444,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
