摘要
目的:建立HPLC法测定人血浆中缬沙坦浓度的方法。方法:以厄贝沙坦为内标,血浆用液液萃取处理。采用AgilentZORBAXSBC18(150mm×4.6mm,5μm);柱温:室温;流动相:乙腈水磷酸三乙胺为40601.01.5(v/v);流速1.0ml·min-1;荧光检测激发波长为265nm,发射波长为378nm。结果:血浆内源性杂质对样品测定无干扰。缬沙坦最低定量浓度为25ng·ml-1,在25～2500ng·ml-1范围内线性关系良好(r=0.9996)。缬沙坦的日内、日间RSD均小于8%,样品多次冻融及提取后24h内稳定性良好。结论:该法快速、灵敏、准确,可用于缬沙坦的体内分析及临床药学研究。
AIM： To establish a high performance liquid chromatography （HPLC） method to determine the concentration of valsartan in human plasma. METHODS： Using lrbesartan as internal standard, valsartan in plasma samples was determined by HPLC with liquid-liquid extraction, achieved by the column of Agilent ZORBAXSB-C18 （150mm×4.6mm, 5μm） at room temperature. The mobile phase consisted of a mixture acetonitrile ： water ： phosphoric acid ： triethylamine was the ratio of 40 ： 60 ： 1.0 ： 1.5 （v/v）, pumped at a flow rate of 1.0 ml·min^-1, the wavelengths of fluorimetric excitation and emission were set at 265 and 378 nm respectively. RESULTS： The drug-free plasma did not interfere with the determination of drugs and internal standard. There was good linear relationships （1/C^2 weighted） between peak area ratio of valsartan to internal standard and C （r=0.9996） within the range of 25-2500 ng·ml^-1. The precision of within-day and between-day was good. The lower limit of quantification was 25 ng·ml^-1 The analytes reconstituted in the mobile phase were also stable at ambient conditions for at least 24h. Furthermore, valsartan was stable for at least three freeze thaw cycles. CONCLUSION： The HPLC method can be used to determine the concentration of valsartan in human plasma.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第9期1038-1040,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
