摘要
目的研究碱性成纤维细胞生长因子(basic fibrob-last growth factor,bFGF)和转化生长因子-β(transforming growth factor-β,TGF-β)对人巩膜成纤维细胞的生长调控,进一步明确bFGF和TGF-β影响近视的作用部位及可能机制。方法取角膜移植后的3只健康供体眼球,分离培养巩膜成纤维细胞并建立细胞系,取第3～5代生长良好的细胞,在培养液中分别加入1ng/ml、3ng/ml、10ng/ml、30ng/ml、100ng/ml、300ng/ml的bFGF以及0.1ng/ml、0.3ng/ml、1ng/ml、3ng/ml、10ng/ml、30ng/ml的TGF-β,两组均设空白对照。6d后进行细胞计数,选用t-test进行统计分析。每株细胞重复3次,共重复3株细胞,观察bFGF和TGF-β对巩膜成纤维细胞的生长调控作用。结果bFGF能明显促进人巩膜成纤维细胞的生长,随浓度增加呈明显剂量效应关系。bFGF大于10ng/ml时(包括10ng/ml),和无bFGF的正常培养液比较,差异具有显著性(P<0.05)。TGF-β能明显抑制人巩膜成纤维细胞的生长,抑制细胞生长的作用亦呈明显的剂量效应关系。TGF-β大于0.3ng/ml时(包括0.3ng/ml),和无TGF-β的正常培养液比较,差异具有显著性(P<0.05)。结论本实验结果显示,bFGF对人巩膜成纤维细胞生长具有明显促进作用,而TGF-β则表现为明显抑制作用。进一步证实外源性bFGF和TGF-β可能作用于巩膜组织,并通过调控巩膜成纤维细胞的生长及巩膜细胞外基质合成代谢单独或协同作用参与巩膜重塑过程。
Objective To study the regulation growth of the basic fibroblast growth factor and transforming growth factor-β on cultured human scleral fibroblasts （HSF） in order to clarify the role of basic fibroblast growth factor （bFGF） and transforming growth factor-β（TGF-β） during myopia development. Methods Three healthy donor eyes after corneal transplantation were used to estab-lish fibroblast cell lines. The third or fifth generation cells which grew well in the culture medium was used. Basic-FGF and TGF-β were tested for six days by 1 ng/ml, 3 ng/ml, 10 ng/ml, 30 ng/ml, 100 ng/ml, 300 ng/ml of bFGF and 0.1 ng/ml, 0.3 ng/ml, 1 ng/ml, 3 ng/ml, 10 ng/ml, 30 ng/ml of TGF-β. The cells were counted after six days and the t-test was used for statistical analysis. Each strain of cells was examined three times for the regulation of TGF-β and bFGF in scleral fibroblast growth. Results Basic- FGF could induce dose-dependent, stimulation the proliferation of HSF. Compared with the blank group, the dose of more than 10 ng/ml bFGF （including 10 ng/ml） was statistically significant increased the proliferation of HSF （P〉0.05）. TGF-β could induce dose-dependent, inhibition the proliferation of HSF. Compared with the blank group, the dose of more than 0.3 ng/ml （including 0.3 ng/ml） TGF-β was statistically significant （P〉0.05） inhibited the proliferation of HSF （P〉0.05）. Conclusion The results show that bFGF has an obvious promoted role on cultured human seleral fibroblast growth while TGF-β can significantly inhibit the growth. And it confirms the possible role of bFGF and TGF-β in the control of the growth of cells and extracellular matrix synthesis alone or synergistically which involved in the sclera remodeling process during myopia development.
出处
《眼视光学杂志》
2008年第3期186-189,共4页
Chinese Journal of Optometry & Ophthalmology
基金
国家自然科学基金项目(30371507
30500549)
浙江省自然科学基金项目(302095
R205739)
浙江省科技厅科技攻关计划-重点科研项目--社会发展项(2003C23005)
浙江省卫生厅重大课题(2002ZD009
2004QN020)
