摘要
目的:探讨生长因子bFGF-2、TGF-β在兔眼外肌术后愈合过程中的表达变化与炎症反应的关系。方法:实验白兔,右眼采用巩膜固定缝线术,左眼作为正常对照,采用免疫组织化学技术和图像分析技术,对实验白兔术后不同愈合时间生长因子在眼外肌创缘组织和正常组织中的表达变化进行研究。结果:实验组bFGF-2、TGF-β表达呈现不同规律,bFGF-2细胞因子在3 h组中表达开始增强,6 h达高峰后一直持续到24 h,在7、14 d组中表达开始下降,但仍高于正常组织中的表达。TGF-β在3 h组中表达开始升高,没有递减趋势,在24 h、71、4 d组中表达持续升高,粘连程度越重表达也越高。对照组中bFGF-2、TGF-β仅有微弱表达。结论:生长因子bFGF-2、TGF-β不仅在眼外肌术后伤口的愈合过程中发挥重要作用,而且在术后炎症反应、瘢痕的形成中也发挥着关键作用。
Objective: Study the relationship between the expression variety of the growth factor bFGF - 2 and TGF-β and inflammatory reaction. Method: Experiment white rabbit right eye in fadenoperation, left eye as normal control. Use technique of immnnohistochemistry and image, study the express variety of grouth factor in wound after different time and normal tissue. Results: bFGF - 2 and TGF-β expression different regularity, the positive of bFGF - 2 significantly in 3 hours, the peak level of bFGF- 2 expression was observed from 6 hours to 24 hours, then decreased in the group of 7days and 14days, but its expression is still higher than normal organization, The positive of TGF-β significantly in 3 hours, but have no gradual decreased trendency, the positive of TGF-β increased in the group of 24 hours and 7 days and 14 days gradually. The more significant of positive expressd the more hard of inflammatory reacted. Thepositive of bFGF - 2 and TGF -β weaked in the control group. Conlusion: Growth factor educe important effect not only in healable wound, but also in inflammation and cicatrice.
出处
《内蒙古医学杂志》
2008年第1期39-41,F0004,共4页
Inner Mongolia Medical Journal