摘要
目的:探讨微波辐照对人鼻咽癌细胞丙二醛(Malondialdehyde,MDA)含量和超氧化物歧化酶(Superoxide Dis-mutase,SOD)活性的影响。方法:运用微波理疗仪以连续波、垂直极化照射方式对人鼻咽癌细胞CNE-2进行微波辐射(频率2450MHz)。实验分为4组:空白对照组,10、20、30mW/cm2微波组。采用TBA显色法和改良NBT羟胺法检测微波辐照对人鼻咽癌细胞MDA含量和SOD活性的影响。结果:随着微波剂量的啬,MDA含量较对照组均呈上升趋势,20和30mW/cm2辐照组MDA含量分别为对照组的1.4和3.5倍,两辐照组与对照组相比差异显著(P<0.05);辐照组SOD活性分别为对照组的68.2%、46.2%和36.0%,10mW/cm2与对照组相比差异显著(P<0.05),20和30mW/cm2辐照组与对照组相比差异极显著(P<0.01)。结论:微波辐照可致细胞MDA含量明显增高,SOD活性降低。
Objective： To study the effect of Microwave Radiation on MDA levels and SOD activity of Nasopharyngeal carcinoma cells. Methods：The expriement were divided into four groups, control group, 10,20and 30 mW/cm^2 groups. The TAB and NBT methods were used to detect the effect of Microwave Radiation on MDA levels and SOD activity of nasopharyngeal carcnoma cells. Results：With increasment of microwave radiation, TAB assay showed that microwave Radiation increased MAD levels 1.4 and 3.5 fold in 20 and 30 mV/cm^2 groups,which were 68. 20%,46. 20% and 36.0% in 20 and 30vW/cm^2 groups,which were statistical significance compared with control group （P〈0.01）. Conclusion：Microwave Radiation could promoted MAD levels and reduced SOD activity.
出处
《中国伤残医学》
2008年第3期8-10,共3页
Chinese Journal of Trauma and Disability Medicine
基金
兰州军区人才培养基金项目(编号:1011)
关键词
微波辐照
人鼻咽癌细胞
MDA
SOD
凋亡
Microwave Radiation
Nasopharyngeal carcinoma cell
MAD
SOD
Apoptosis
