摘要
目的：研究地塞米松（DEX）对体外培养人牙周膜成纤维（PDLF）细胞增殖的影响，寻找体外培养人PDLF（hPDLF）细胞的优化条件，为牙周组织再生的研究工作奠定基础。方法：体外分离培养的hPDLF细胞随机分为5组，分别用5种含10％胎牛血清的DMEM细胞培养基培养5～8代：①空白对照组（无DEX）；②含5mg·L^-1 DEX；③含10mg·L^-1 DEX；④含20mg·L^-1 DEX；⑤含50mg·L^-1 DEX。以MTT法测定DEX对hPDLF细胞增殖率的影响；采用倒置相差显微镜观察hPDLF细胞在添加不同浓度DEX的胎牛血清培养基中培养后的细胞形态学改变。结果：将hPDLF细胞接种于24孔板后的第3天，细胞出现汇合现象。细胞在孔底呈鳞片状。采用DEX条件DMEM细胞培养液培养，可见细胞逐渐变成多层，细胞形态变小，突起变钝。MTT法检测结果表明含不同浓度DEX的DMEM培养基中培养的hPDLF细胞数均高于对照组（P〈0．05）；不同浓度的DEX对hPDLF细胞增殖均有影响（P〈0．05），且随着浓度增加，hPDLF细胞增殖能力逐渐增高。结论：DEX能使体外培养的hPDLF细胞表现很强的增殖活性。
Objective To investigate the effect of dexamethasone on proliferation of human periodontal ligament fibroblasts （hPDLF） cultivated in vitro, and search for optimal culture condition for hPDLF in vitro, and provide basis for further study on regeneration of periodontinum. Methods The hPDLF cultivated in vitro were divided into 5 groups and cultivated in 5 different culture media separately which all included 10% fetal bovine serium： ①control group （no DEX）; ②5 mg·L^-1 DEX; ③10 mg·L^-1 DEX; ④20 mg·L^-1 DEX; ⑤50 mg·L^-1 DEX. The proliferation of hPDLF was assyed by MTT method. The phase contrast microscope was used to observe the morphological changes of the cells. Results The PDLF appeared aggregation and cells on bottom of culture bottle showed squamae-shape after inoculated on plate with 24 hole for 3 d. There were more layers of smaller hPDLF with blunt prominency came forth after cultivated in DEX culture media. MTT method showed that DEX promoted the proliferation of hPDLF obviously compared with control group （P〈0.05）. The different concentrations of DEX had different effects on proliferation of hPDLF （P〈0.05）, the proliferation ability was enhanced with the increasing of DEX doses. Conclusion DEX could improve the proliferation ability of hPDLF cultivated in vitro.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第3期462-465,F0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(20020616)
关键词
人牙周膜成纤维细胞
体外培养
胎牛血清
地塞米松
human periodontal ligament fibroblasts
culture in vitro
fetal bovine serium
dexamethasone
