摘要
目的:观察阿糖胞苷(Ara-C)对急性白血病细胞CD86分子表达的影响,并探讨其作用机制。方法:流式细胞术(FCM)检测U937、HL60、NB4细胞经Ara-C处理前后CD86分子表达变化,RT-PCR检测Ara-C对各组细胞CD86mRNA、NF-κB以及细胞因子IFN-γ的表达变化。结果:经Ara-C处理的急性白血病细胞CD86分子表达与对照组相比均明显升高(P<0.05);CD86mRNA表达水平也明显增强;Ara-C处理后细胞核内NF-κB表达明显上调;并且IFN-γmRNA在T细胞活化72h可检测到。结论:Ara-C能使U937、HL60、NB4急性白血病细胞CD86表达增加,有利于NF-κB等转录因子活化,促进CD86转录增强、表达增加并可有效地增强肿瘤细胞的免疫原性,激活T细胞,B7-2在T细胞活化中起着更重要的作用。
AIM： To observe the effects of Ara-c on the expression of CD86 molecule on acute leukemia cells and explore the possible mechanisms. METHODS： The expression of CD86 on U937, HL-60 and NB4 cell lines treated with or without Ara-C wa assayed by flow cytometry, The mRNA expression of CD86, NF-κB, IFN-γ was examined by semiquantitative RT-PCR, RESULTS： UP-regulation of CD86 was observed on those cells treated by Are-c, The leve of CD86 and NF-κB mRNA in Ara-c treated cells was significantly enhanced, IFN-γ was detectable 72 hours after T cell activation, CONCLUSION： Ara-C can enhance CD86 and NF-κB expression on acute leukemia cells, which may play critical role in T cell activation and differentiation.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第6期550-552,556,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30400558
30570772)
