摘要
目的观察丁酸钠(SB)对急性白血病细胞共刺激分子表达的影响,并探讨其分子机制。方法以流式细胞术检测NB4、HL-60、Kasumi1、U937和Jurkat细胞经SB处理前、后CD86、CD80分子表达,同种混合淋巴细胞反应观察免疫调节功能,NFκB试剂盒检测SB对核内NFκB含量的影响。结果经SB处理后,各白血病细胞株细胞CD86和CD80分子表达与对照组比较均出现不同程度上调,其中0.5mmol/LSB处理的NB4细胞组较空白对照组CD86阳性细胞率增高36.8倍,NF-κB含量增加与CD86分子上调相一致,混合淋巴细胞反应显示SB处理的细胞可明显刺激异基因淋巴细胞增殖。结论SB可上调急性白血病细胞共刺激分子CD86和CD80,NF-κB为其参与上调CD86分子的重要的转录因子。
Objective To explore the effect of sodium butyrate(SB) on the expression of costimulatory molecules in acute leukemia cells and its mechanism. Methods The expression of CD86 and CD80 was examined on the surfaces of NB4,HL-60,Kasumi-1,U937 and Jurkat cells by flow cytometric analysis after treated by SB or not. Allogeneic mixed lymphocyte reaction was used to evaluate the immunomodulatory effects of cells treated by SB. Activated NF-κB was measured with an NF-кB assay kit. Results Up-regulation of CD86 and CD80 at various levels was observed on these leukemia cells treated by SB. The ratio of CD86 expressing cell in NB4 cells treated by 0.5mmol/L SB was 36.8 times higher than that in control. Up-regulation of NF-κB was similar to that of CD86. Allogeneic lymphocyte proliferation was strongly stimulated by the SB treated cells. Conclusion SB can improve the expression of CD86 in acute leukemia cells. NF-κB was an important transcription factor involved in the up-regulation of CD86.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2005年第5期265-268,共4页
Chinese Journal of Hematology
基金
吉林省计委资助项目(20031268)
