摘要
目的寻找重型脑损伤（severe traumatic brain injury，sTBI）大鼠伤后不同时间的蛋白质组变化规律。方法提取创伤后3，7和14d的鼠脑组织总蛋白进行双向凝胶电泳，筛选和鉴定差异蛋白质点。结果共筛选出17个差异蛋白质点，它们参与细胞代谢、应激与炎症反应等过程。结论利用双向凝胶电泳技术可寻找到多种参与重型脑损伤病程演进的蛋白质。
Objective To study the changes of proteome expression in brain tissues from rats with severe traumatic brain injury （sTBI）. Methods Total protein of brain tissues were obtained at days 3, 7 and 14 for two-dimensional gel electrophoresis to screen and identify differential protein spots. Results We screened 17 differential protein spots that were involved in cellular metabolism, stress and inflammatory reaction. Conclusion Some differential proteins involved in sTBI can be found by twodimensional gel electrophoresis.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2008年第6期425-427,共3页
Chinese Journal of Trauma
基金
天津市科委攻关课题资助项目（06YFSZSF01200）
关键词
脑损伤
双向凝胶电泳技术
蛋白质组
Brain injuries
Two-dimensional gel eleetrophoresis
Proteome
