摘要
目的应用表面加强激光解吸离子化飞行时间质谱（surface enhanced lase rdesorptionionization time-of-fight mass spectrometry，SELDI-TOF-MS）技术筛选重型颅脑创伤（severe traumatic brain injury，sTBI）患者早期血清差异表达蛋白，初步建立sTBI患者早期血清蛋白诊断指纹图谱。方法获取临床收住院早期未接受药物干预治疗的重型颅脑创伤患者（GCS3～8分）与健康体检者血清样本，根据从创伤到入住院时间间隔将病人血清分为≤1h、1-3h和〉3-8h组，分别混合各组血清样本取消个体差异，利用弱阳离子芯片CM10经质谱分析血清中差异表达蛋白，根据检测结果初步分析建立sTBI病人早期血清蛋白诊断指纹图谱。结果样品血清中共获得71个有效蛋白质峰，有7个峰与对照组比较有差异，其中m／z4210、m／z7116和m／z7320在3组病人中均显著升高（P〈0．05）；m／z4977和m／z8719在3组中均显著降低（P〈0．05）；m／z4679的升高出现在1-3h和〉3-8h组；m／z9431的升高只出现〉3-8h组。结论应用SELDI-TOF-MS技术可寻找到多种血清差异蛋白表达，这些蛋白可能参与sTBI患者早期分子病理事件，它们的发现将会为临床药物筛选和（或）新药物开发提供重要参考。
Objective To seek serum biomarkers and establish serum protein fingerprint in early severe traumatic brain injury patients by surface enhanced laser desorption-ionization time-of-fight mass spectrometry. Methods Serum samples were obtained from 86 cases of early untreated traumatic brain injury patients（ GCS scores of 3 to 8） and 50 cases healthy people. Three groups （ ≤ 1h, 1-3h and 〉 3-8h） were determined by the intervals from injury occur to admission. Serum samples were admixed to eliminate individual variation. Protein chip CM10 and SELDI-TOF-MS were used to detect serum differential expression proteins, and establish diagnostic fingerprint of early traumatic brain injury patients. Results 71 protein peaks were detected in serum samples, m/z 4210, m/z 7116 and m/z 7320 in all three groups were significantly higher than control group, but m/z 4977 and m/z 8719 were lower, m/z 4679 was increased in 1-3h and 〉 3-8h groups, and m/z 9431 increased only in 〉 3-8h group. Conclusion Some differential expression proteins can be found by SELDI-TOF-MS. These proteins maybe involve in early pathologic molecular events, and direct clinical drug screening and/or new drug development.
出处
《中华神经外科杂志》
CSCD
北大核心
2008年第2期105-108,共4页
Chinese Journal of Neurosurgery
基金
天津市科委攻关课题基金（06YFSZSF01200）
关键词
颅脑损伤
蛋白质组
光谱法
质量
基质辅助激光解析电离
Craniocerebral trauma
Proteome
Spectrometry, mass, matrix-assisted laser desorption
